@article{27342368fa7c4d00a8f58c632f288cad,
title = "Carcinogen-mediated induction of SV40 DNA synthesis in SV40 transformed chinese hamster embryo cells",
abstract = "Exposure of SV40-transformed Chinese hamster embryo cells (line CO50) to a series of physical and chemical carcinogens (including activation-dependent and activation-independent varieties) resulted in the induction of viral DNA synthesis. The carcinogen mediated amplification of SV40 DNA was demonstrated by a highly sensitive in situ hybridization procedure for the detection of cells synthesizing SV40 DNA. Treatment of CO50 cells with an inhibitor of polycyclic hydrocarbon metabolism (7,8-benzoflavone) prior to the application of benzo[a]pyrene or 7,12-dimethyl-benz[a]anthracene prevented the induction of SV40 DNA synthesis, indicating that the induction depends upon the metabolic activation of these compounds. Non-carcinogenic hydrocarbons were inactive under this assay. Two different protocols for determining the inducing potential of a compound are presented. The properties of this test and its possible use as a short-term assay for potential carcinogens is discussed. The possibility that the induction of SV40 DNA synthesis is a reflection of a general gene amplification phenomenon mediated by carcinogens is discussed.",
author = "Sara Lavi and Sara Etkin",
note = "Funding Information: We thank E.Winocour for his support and encouragement and T.Vogel for helpful discussions. This work was supported in part by grants from the Leukemia Research Foundation, Chicago, the National Council for Research and Development, Israel and DKFZ, Heidelberg, Germany. S.L. is a Leukemia Society of America scholar.",
year = "1981",
doi = "10.1093/carcin/2.5.417",
language = "אנגלית",
volume = "2",
pages = "417--423",
journal = "Carcinogenesis",
issn = "0143-3334",
publisher = "Oxford University Press",
number = "5",
}