Caloric restriction ameliorates cardiomyopathy in animal model of diabetes

Keren Cohen, Maayan Waldman, Nader G. Abraham, Michal Laniado-Schwartzman, Danny Gurfield, Dan Aravot, Michael Arad, Edith Hochhauser*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background The db/db mouse is an animal model of diabetes in which leptin receptor activity is deficient resulting accelerated cardiomyopathy when exposed to angiotensin (AT). Toll-like receptors 4 and 2 (TLR4, TLR2) are pattern recognition receptors, that recognize pathogen-associated molecular patterns and exacerbate and release inflammatory cytokines. Fetuin A (Fet A) is a fatty acid carrier which affects inflammation and insulin resistance in obese humans and animals through TLRs. The aim of this study was to investigate the effect of caloric restriction (CR) on free fatty acids (FFA) level and the inflammatory response in diabetic cardiomyopathy. Methods and results Left ventricular hypertrophy, increased fibrosis and leukocytes infiltration were observed in db/db AT treated hearts. Serum glucose, FFA, and cholesterol levels were elevated in db/db AT treated mice. Cardiac expression of PPARα increased while AKT phosphorylation was decreased. Conclusions Cumulatively, CR elevated cardiac PPARα improved the utilization of fatty acids, and reduced myocardial inflammation as seen by reduced levels of Fet A. Thus CR negated cardiomyopathy associated with AT in an animal model of diabetes suggesting that CR is an effective therapeutic approach in the treatment of diabetes and associated cardiomyopathy.

Original languageEnglish
Pages (from-to)147-153
Number of pages7
JournalExperimental Cell Research
Volume350
Issue number1
DOIs
StatePublished - 1 Jan 2017

Keywords

  • AKT
  • Caloric restriction
  • Fetuin-A
  • Free fatty acids
  • PPARα
  • TLR2
  • TLR4
  • TNF-α
  • db/db mice

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