TY - JOUR
T1 - Bowel ischaemia-reperfusion-induced liver and lung injury
T2 - The fundamental role of eicosanoids and xanthine oxidase-generated oxygen free radicals
AU - Weinbroum, A. A.
AU - Coldman, G.
AU - Kluger, Y.
AU - Hag, M.
AU - Marmour, S.
AU - Sorkine, P.
AU - Klausner, J. M.
AU - Niv, D.
AU - Rudick, V.
PY - 1997
Y1 - 1997
N2 - Objective: To assess complementary induction of remote organ injury following bowel ischaemia-reperfusion by eicosanoids and xanthine oxidase. Design: Randomized, controlled, animal study. Setting: Hospital-based animal research facility. Materials: 40 rats in 5 groups. Interventions: Sham laparotomy, laparotomy and superior mesenteric artery occlusion (ischaemia 1h) and reperfusion (3h) or laparotomy and arterial occlusion and reperfusion in rats treated with thromboxane synthase inhibitor OKY-046, leukotrienes synthase inhibitor diethylcarbamazine or xanthine oxidase inhibitor allopurinol. Main outcome measures: Bowel, liver and lung wet: dry weight ratio, tissue: circulation albumin I125 permeability index, polymorphonuclear neutrophils tissue counts. Results: Ischaemia intestinal and lungs wet: dry ratios increased by 50% over sham or treatment groups; liver ratios were similar. Albumin indexes in ischaemia organs were 1.5-3 times more than shams and treatment values, and allopurinol normalized it best. Neutrophils counts in all ischaemia organs were double that of shams; allopurinol prevented sequestration completely, eicosanoids inhibitors did so partially. Conclusions: Xanthine oxidase initiated remote organ injury following bowel ischaemia-reperfusion. Allopurinol prevented it entirely while eicosanoids inhibitors only partially.
AB - Objective: To assess complementary induction of remote organ injury following bowel ischaemia-reperfusion by eicosanoids and xanthine oxidase. Design: Randomized, controlled, animal study. Setting: Hospital-based animal research facility. Materials: 40 rats in 5 groups. Interventions: Sham laparotomy, laparotomy and superior mesenteric artery occlusion (ischaemia 1h) and reperfusion (3h) or laparotomy and arterial occlusion and reperfusion in rats treated with thromboxane synthase inhibitor OKY-046, leukotrienes synthase inhibitor diethylcarbamazine or xanthine oxidase inhibitor allopurinol. Main outcome measures: Bowel, liver and lung wet: dry weight ratio, tissue: circulation albumin I125 permeability index, polymorphonuclear neutrophils tissue counts. Results: Ischaemia intestinal and lungs wet: dry ratios increased by 50% over sham or treatment groups; liver ratios were similar. Albumin indexes in ischaemia organs were 1.5-3 times more than shams and treatment values, and allopurinol normalized it best. Neutrophils counts in all ischaemia organs were double that of shams; allopurinol prevented sequestration completely, eicosanoids inhibitors did so partially. Conclusions: Xanthine oxidase initiated remote organ injury following bowel ischaemia-reperfusion. Allopurinol prevented it entirely while eicosanoids inhibitors only partially.
KW - Bowel
KW - Ischaemia-reperfusion
KW - Leukotriene
KW - Oxygen free radicals
KW - Remote organ injury
KW - Thromboxane
KW - Xanthine oxidase
UR - http://www.scopus.com/inward/record.url?scp=0031444127&partnerID=8YFLogxK
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AN - SCOPUS:0031444127
SN - 1234-1010
VL - 3
SP - 624
EP - 630
JO - Medical Science Monitor
JF - Medical Science Monitor
IS - 5
ER -