@article{994ea972b814476891f0eec802d91fee,
title = "Boric acid reversibly inhibits the second step of pre-mRNA splicing",
abstract = "Several approaches have been used to identify the factors involved in mRNA splicing. None of them, however, comprises a straightforward reversible method for inhibiting the second step of splicing using an external reagent other than a chelator. This investigation demonstrates that the addition of boric acid to an in vitro pre-mRNA splicing reaction causes a dose-dependent reversible inhibition effect on the second step of splicing. The mechanism of action does not involve chelation of several metal ions; hindrance of 3′ splice-site; or binding to hSlu7. This study presents a novel method for specific reversible inhibition of the second step of pre-mRNA splicing.",
keywords = "Boric acid, Inhibition, Pre-mRNA splicing, Spliceosome",
author = "Noam Shomron and Gil Ast",
note = "Funding Information: This work was supported by an award from the Israeli Science Foundation and in part by a grant from the Israel Cancer Association and the MOP Israel–India to G.A. N.S. was partially supported by a Rieger Foundation scholarship. We would also like to thank Prof. Joseph Sperling for his helpful discussion and Leeat Anker for commenting on the manuscript.",
year = "2003",
month = sep,
day = "25",
doi = "10.1016/S0014-5793(03)00928-1",
language = "אנגלית",
volume = "552",
pages = "219--224",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "John Wiley and Sons Inc.",
number = "2-3",
}