Boric acid reversibly inhibits the second step of pre-mRNA splicing

Noam Shomron, Gil Ast*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Several approaches have been used to identify the factors involved in mRNA splicing. None of them, however, comprises a straightforward reversible method for inhibiting the second step of splicing using an external reagent other than a chelator. This investigation demonstrates that the addition of boric acid to an in vitro pre-mRNA splicing reaction causes a dose-dependent reversible inhibition effect on the second step of splicing. The mechanism of action does not involve chelation of several metal ions; hindrance of 3′ splice-site; or binding to hSlu7. This study presents a novel method for specific reversible inhibition of the second step of pre-mRNA splicing.

Original languageEnglish
Pages (from-to)219-224
Number of pages6
JournalFEBS Letters
Issue number2-3
StatePublished - 25 Sep 2003


  • Boric acid
  • Inhibition
  • Pre-mRNA splicing
  • Spliceosome


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