Boric acid reversibly inhibits the second step of pre-mRNA splicing

Noam Shomron; Gil Ast

doi:10.1016/S0014-5793(03)00928-1

Boric acid reversibly inhibits the second step of pre-mRNA splicing

Noam Shomron, Gil Ast^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Several approaches have been used to identify the factors involved in mRNA splicing. None of them, however, comprises a straightforward reversible method for inhibiting the second step of splicing using an external reagent other than a chelator. This investigation demonstrates that the addition of boric acid to an in vitro pre-mRNA splicing reaction causes a dose-dependent reversible inhibition effect on the second step of splicing. The mechanism of action does not involve chelation of several metal ions; hindrance of 3′ splice-site; or binding to hSlu7. This study presents a novel method for specific reversible inhibition of the second step of pre-mRNA splicing.

Original language	English
Pages (from-to)	219-224
Number of pages	6
Journal	FEBS Letters
Volume	552
Issue number	2-3
DOIs	https://doi.org/10.1016/S0014-5793(03)00928-1
State	Published - 25 Sep 2003

Keywords

Boric acid
Inhibition
Pre-mRNA splicing
Spliceosome

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10.1016/S0014-5793(03)00928-1

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abstract = "Several approaches have been used to identify the factors involved in mRNA splicing. None of them, however, comprises a straightforward reversible method for inhibiting the second step of splicing using an external reagent other than a chelator. This investigation demonstrates that the addition of boric acid to an in vitro pre-mRNA splicing reaction causes a dose-dependent reversible inhibition effect on the second step of splicing. The mechanism of action does not involve chelation of several metal ions; hindrance of 3′ splice-site; or binding to hSlu7. This study presents a novel method for specific reversible inhibition of the second step of pre-mRNA splicing.",

keywords = "Boric acid, Inhibition, Pre-mRNA splicing, Spliceosome",

author = "Noam Shomron and Gil Ast",

note = "Funding Information: This work was supported by an award from the Israeli Science Foundation and in part by a grant from the Israel Cancer Association and the MOP Israel–India to G.A. N.S. was partially supported by a Rieger Foundation scholarship. We would also like to thank Prof. Joseph Sperling for his helpful discussion and Leeat Anker for commenting on the manuscript.",

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AU - Ast, Gil

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N2 - Several approaches have been used to identify the factors involved in mRNA splicing. None of them, however, comprises a straightforward reversible method for inhibiting the second step of splicing using an external reagent other than a chelator. This investigation demonstrates that the addition of boric acid to an in vitro pre-mRNA splicing reaction causes a dose-dependent reversible inhibition effect on the second step of splicing. The mechanism of action does not involve chelation of several metal ions; hindrance of 3′ splice-site; or binding to hSlu7. This study presents a novel method for specific reversible inhibition of the second step of pre-mRNA splicing.

AB - Several approaches have been used to identify the factors involved in mRNA splicing. None of them, however, comprises a straightforward reversible method for inhibiting the second step of splicing using an external reagent other than a chelator. This investigation demonstrates that the addition of boric acid to an in vitro pre-mRNA splicing reaction causes a dose-dependent reversible inhibition effect on the second step of splicing. The mechanism of action does not involve chelation of several metal ions; hindrance of 3′ splice-site; or binding to hSlu7. This study presents a novel method for specific reversible inhibition of the second step of pre-mRNA splicing.

KW - Boric acid

KW - Inhibition

KW - Pre-mRNA splicing

KW - Spliceosome

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Boric acid reversibly inhibits the second step of pre-mRNA splicing

Abstract

Keywords

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