Bone Marrow–Derived Macrophage (BMDM ) Infection by Listeria monocytogenes

Etai Boichis, Shai Ran Sapir, Anat A. Herskovits

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Listeria monocytogenes is a gram-positive bacterium adapted to life as both an environmental saprophyte and a pathogenic parasite of mammalian hosts, with a transcriptomic program tailored for each niche. Study of the L. monocytogenes pathogenic lifestyle requires conditions that mimic the mammalian niche. Of the myriad experimental models used to achieve such conditions, the bone marrow–derived macrophage (BMDM) is a relatively simple and reliable primary immune cell model for L. monocytogenes infections. Here we describe the extraction, preparation, and storage of BMDMs and their use in L. monocytogenes infection experiments.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages83-93
Number of pages11
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2427
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Bacterial infection
  • Bone marrow derived macrophages
  • Intracellular growth
  • Listeria monocytogenes

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