Bone marrow Minimal Disseminated Disease (MDD) and Minimal Residual Disease (MRD) in childhood T-cell lymphoblastic lymphoma stage III, detected by Flow Cytometry (FC) and Real-Time Quantitative Polymerase Chain Reaction (RQ-PCR)

Batia Stark, Smadar Avigad, Drorit Luria, Sigal Manor, Tali Reshef-Ronen, Gali Avrahami, Isaac Yaniv

Research output: Contribution to journalArticlepeer-review

Abstract

Background. Despite overlapping features of T-cell lymphoblastic lymphoma (T-LLy) and T-cell acute lymphoblastic leukemia (T-ALL), which respond favorably to T-ALL treatment, clinical and biological differences exist. We retrospectively assessed the prevalence of submicroscopic bone marrow (BM) minimal disseminated disease (MDD) at diagnosis and the early response to treatment (minimal residual disease - MRD) and their prognostic significance in 17 children with stage III T-LLy treated according to Berlin-Frankfurt-Munster (BFM) non-Hodgkin lymphoma protocols. Procedure. Four-color flow cytometry (FC) was used for lymphoma associated immunophenotype and real-time quantitative polymerase chain reaction (RQ-PCR) for T-cell receptor (TCR β/Δ/ γ) gene rearrangements with at least 0.01% sensitivity. Results. Two markers per patient were identified in all cases using FC and in 80% using RQ-PCR. BM MDD at diagnosis of ≥0.01% was detected by FC and RQ-PCR in 88% and 80% of patients, respectively, and by at least one of the methods in all patients. A significant correlation was achieved between the methods by Pearson correlation analysis (P = 0.004). MRD levels significantly decreased to very low levels on day 33 in 9 out of 10 patients studied. The only patient that remained positive relapsed. Conclusions. MDD was prevalent in stage III T-LLy, for which we could not prove a prognostic significance in the context of ALL-like treatment. This study shows that both FC and RQ-PCR methods are efficient for MDD and MRD analyses in T-LLy.

Original languageEnglish
Pages (from-to)20-25
Number of pages6
JournalPediatric Blood and Cancer
Volume52
Issue number1
DOIs
StatePublished - Jan 2009

Keywords

  • Flow cytometry
  • Minimal residual disease
  • PCR
  • T-lymphoblastic lymphoma

Fingerprint

Dive into the research topics of 'Bone marrow Minimal Disseminated Disease (MDD) and Minimal Residual Disease (MRD) in childhood T-cell lymphoblastic lymphoma stage III, detected by Flow Cytometry (FC) and Real-Time Quantitative Polymerase Chain Reaction (RQ-PCR)'. Together they form a unique fingerprint.

Cite this