TY - JOUR
T1 - Blot analyses of glycoconjugates
T2 - Enzyme-hydrazide-a novel reagent for the detection of aldehydes
AU - Gershoni, Jonathan M.
AU - Bayer, Edward A.
AU - Wilchek, Meir
PY - 1985/4
Y1 - 1985/4
N2 - A procedure for the general staining of glycoproteins and other glycoconjugates on protein blots has been developed. Aldehydes are formed on the sugars of glycoconjugates by periodate oxidation which then react with hydrazide groups of enzyme-hydrazides, a novel reagent designed for aldehyde detection. The bound enzyme-hydrazide is demonstrated histochemically. The new assay is advantageous over periodic-acid Schiff staining of gels as its reagents and signals are stable and the process is simple and expedient, and provides greater sensitivity.
AB - A procedure for the general staining of glycoproteins and other glycoconjugates on protein blots has been developed. Aldehydes are formed on the sugars of glycoconjugates by periodate oxidation which then react with hydrazide groups of enzyme-hydrazides, a novel reagent designed for aldehyde detection. The bound enzyme-hydrazide is demonstrated histochemically. The new assay is advantageous over periodic-acid Schiff staining of gels as its reagents and signals are stable and the process is simple and expedient, and provides greater sensitivity.
KW - enzyme-hydrazide
KW - glycoconjugates
KW - glycoprotein
KW - periodic acid Schiff stain
KW - protein blots
UR - http://www.scopus.com/inward/record.url?scp=0021959044&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(85)90395-1
DO - 10.1016/0003-2697(85)90395-1
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0021959044
SN - 0003-2697
VL - 146
SP - 59
EP - 63
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -