Blot analyses of glycoconjugates: Enzyme-hydrazide-a novel reagent for the detection of aldehydes

Jonathan M. Gershoni, Edward A. Bayer, Meir Wilchek

Research output: Contribution to journalArticlepeer-review

Abstract

A procedure for the general staining of glycoproteins and other glycoconjugates on protein blots has been developed. Aldehydes are formed on the sugars of glycoconjugates by periodate oxidation which then react with hydrazide groups of enzyme-hydrazides, a novel reagent designed for aldehyde detection. The bound enzyme-hydrazide is demonstrated histochemically. The new assay is advantageous over periodic-acid Schiff staining of gels as its reagents and signals are stable and the process is simple and expedient, and provides greater sensitivity.

Original languageEnglish
Pages (from-to)59-63
Number of pages5
JournalAnalytical Biochemistry
Volume146
Issue number1
DOIs
StatePublished - Apr 1985
Externally publishedYes

Keywords

  • enzyme-hydrazide
  • glycoconjugates
  • glycoprotein
  • periodic acid Schiff stain
  • protein blots

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