TY - JOUR
T1 - Blastogenic response of lymphocytes derived from patients with hematopoietic malignancies to antigens of a type C retrovirus isolated from a Burkitt's lymphoma cell line
AU - Schwarzbard, Zivia
AU - Gotlieb-Stematsky, Tamar
AU - Vonsover, Ami
AU - Djaldetti, Meir
AU - Hallak, Aharon
AU - Yaniv, Abraham
N1 - Funding Information:
Accepted 28 March 1983. *This research project was supported by a grant from the Israel Cancer Association. §To whom requests for reprints should be addressed.
PY - 1983/10
Y1 - 1983/10
N2 - Cellular immune response to antigens associated with a type C retrovirus derived from Burkitt's lymphoma (BL) lymphoblastoid cells was studied in patients with hematopoietic malignancies, noncancer patients and healthy controls. Repsonse was determined by lymphocyte blastogenesis assay measuring [3H]-thymidine incorporation, thereby enabling the calculation of stimulation indices (SI). Positive response (SI>2;2.0) was demonstrated in patients with multiple myeloma (MM), chronic lymphocytic leukemia (CLL), chronic myelocytic leukemia (CML) and BL. No response was demonstrated in the non-cancer and healthy controls. Specific blastogenic response was obtained towards antigen extracted from three different cell lines infected with the tested type C retrovirus. No response was evident towards purified whole or purified disrupted virions or antigen extracted from murine myeloma MOPC-315 cells secreting a murine type C retrovirus or an antigen extracted from Mason-Pfizer monkey virus-infected NC-37 cells. The correlation between human hematopoietic malignancies and the in vitro lymphocyte blastogenic response to the BL-derived type C retrovirus was statistically highly significant.
AB - Cellular immune response to antigens associated with a type C retrovirus derived from Burkitt's lymphoma (BL) lymphoblastoid cells was studied in patients with hematopoietic malignancies, noncancer patients and healthy controls. Repsonse was determined by lymphocyte blastogenesis assay measuring [3H]-thymidine incorporation, thereby enabling the calculation of stimulation indices (SI). Positive response (SI>2;2.0) was demonstrated in patients with multiple myeloma (MM), chronic lymphocytic leukemia (CLL), chronic myelocytic leukemia (CML) and BL. No response was demonstrated in the non-cancer and healthy controls. Specific blastogenic response was obtained towards antigen extracted from three different cell lines infected with the tested type C retrovirus. No response was evident towards purified whole or purified disrupted virions or antigen extracted from murine myeloma MOPC-315 cells secreting a murine type C retrovirus or an antigen extracted from Mason-Pfizer monkey virus-infected NC-37 cells. The correlation between human hematopoietic malignancies and the in vitro lymphocyte blastogenic response to the BL-derived type C retrovirus was statistically highly significant.
UR - http://www.scopus.com/inward/record.url?scp=0020572067&partnerID=8YFLogxK
U2 - 10.1016/0277-5379(93)90011-S
DO - 10.1016/0277-5379(93)90011-S
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0020572067
SN - 0277-5379
VL - 19
SP - 1411
EP - 1420
JO - European Journal of Cancer and Clinical Oncology
JF - European Journal of Cancer and Clinical Oncology
IS - 10
ER -
