Bird erythrocytes in experimentally introduced blood clots, for differentiation from autogenous mammalian clots

Meir Djaldetti*, Uri Sandbank, Andre de Vries

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

In studies of the effect of snake venom on blood clotting, 5 ml of freshly drawn, unclotted dog blood was centrifuged to separate red cells from plasma. Chicken erythrocytes were added to the plasma to give 2 × 106 cells/ml. The mixture was clotted by adding 1 ml of 0.1 M CaCl2 to it, and clotting allowed to occur in an 8 mm (ID) glass tube. The 10 cm long clot so obtained was injected into the inferior vena cava of the experimental dog. Such introduced clots and their emboli could be readily recognized by the presence of nucleated erythrocytes.

Original languageEnglish
Pages (from-to)211-213
Number of pages3
JournalBiotechnic and Histochemistry
Volume41
Issue number4
DOIs
StatePublished - 1966

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