Bioreactor engineering: Regenerating the dynamic cell microenvironment

Tal Dvir, Smadar Cohen

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


The replacement of the "flat biology" of the Petri dish with three-dimensional (3D) cell cultures has shown to narrow the gap between cell behaviours and function in vitro and at the physiological settings. A fundamental challenge to realise the potential of the 3D cell culture is the design and application of "smart" bioreactor systems. These systems should provide homogenous mass transport into the internal volume of the cultured cell constructs as well as to efficiently propagate physical and mechanical stimuli. Herein, we describe the design principles of various bioreactors, starting with the conventional spinner flasks, the rotary wall vessels and up to the latest perfusion vessels. In particular, the key role of perfusion bioreactors in regenerating the dynamic 3D cell microenvironment is demonstrated by providing a few successful examples of engineering thick functional tissues, such as the cardiac muscle tissue. In closing this chapter, we envision future innovations in bioreactors.

Original languageEnglish
Title of host publicationAdvances in Tissue Engineering
PublisherImperial College Press
Number of pages19
ISBN (Electronic)9781848161832
ISBN (Print)9781848161825
StatePublished - 1 Jan 2008
Externally publishedYes


  • 3D Microenvironment
  • Cardiac Tissue Engineering
  • Interstitial Fluid Flow
  • Mass Transport
  • Mechanical Stress
  • Perfusion Bioreactor


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