Biomonitoring marine environmental quality at the level of gene-expression-testing the feasibility of a new approach

A. Sultan, A. Abelson, V. Bresler, L. Fishelson, O. Mokady

Research output: Contribution to journalConference articlepeer-review

Abstract

Differential-display PCR (DD-PCR), a comparative method for detecting alterations in the expression pattern of unknown genes, was applied to identify markers for environmental pollution of the coastal marine environment, in Donax trunculus (Mollusca: Bivalvia). Bivalves collected from a site typified by industrial pollution were compared with bivalves collected from an unpolluted site. Additionally, bivalves from the unpolluted site were exposed in the laboratory to increasing levels of pollution. Multiple responsive fragments, including qualitative and quantitative differentials, were observed in both experimental designs. Fragment-specific primers may be designed for use in diagnostic amplifications, according to the sequence of selected differential fragments. This study is a first step toward the establishment of a marine warning and monitoring system based on the most basic level of biological response.

Original languageEnglish
Pages (from-to)269-274
Number of pages6
JournalWater Science and Technology
Volume42
Issue number1-2
DOIs
StatePublished - 2000
EventProceedings of the 7th International Conference of the Israel Society for Ecology and Environmental Quality Sciences - Jerusalem, Isr
Duration: 13 Jun 199918 Jun 1999

Keywords

  • Coastal environment
  • DD-PCR
  • Donax trunculus
  • Gene expression
  • Mediterranean
  • Pollution

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