TY - JOUR
T1 - Bioinspired Amyloid Nanodots with Visible Fluorescence
AU - Lapshina, Nadezda
AU - Shishkin, Ivan I.
AU - Nandi, Ramesh
AU - Noskov, Roman E.
AU - Barhom, Hani
AU - Joseph, Sijo
AU - Apter, Boris
AU - Ellenbogen, Tal
AU - Natan, Amir
AU - Ginzburg, Pavel
AU - Amdursky, Nadav
AU - Rosenman, Gil
N1 - Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2019/3/5
Y1 - 2019/3/5
N2 - Nanoscale bioimaging is a highly important scientific and technological tool, where fluorescent (FL) proteins, organic molecular dyes, inorganic quantum dots, and lately carbon dots are widely used as light emitting biolabels. In this work, a new class of visible FL bioorganic nanodots, self-assembled from short peptides of different composition and origin, is introduced. It is shown that the electronic energy spectrum of native nonfluorescent peptide nanodots (PNDs) is deeply modified upon thermally mediated refolding of their biological secondary structure from native metastable to stable β-sheet rich structure. This refolding leads to the appearance of a broadband optical absorption across visible region and tunable, excitation-dependent visible FL of the nanodots with a high quantum yield of ≈30%. It is shown that this intriguing biophotonic effect appears in several peptides/proteins and does not require the presence of aromatic residues. It is assumed that the origin of the phenomenon is related to proton transfer along network of reconstructed intermolecular hydrogen bonds, stabilizing the thermally induced supramolecular β-sheet structure. The biocompatible FL PNDs can be potentially applied as high-resolution bioimaging labels toward advanced biotechnology and biomedical theranostics.
AB - Nanoscale bioimaging is a highly important scientific and technological tool, where fluorescent (FL) proteins, organic molecular dyes, inorganic quantum dots, and lately carbon dots are widely used as light emitting biolabels. In this work, a new class of visible FL bioorganic nanodots, self-assembled from short peptides of different composition and origin, is introduced. It is shown that the electronic energy spectrum of native nonfluorescent peptide nanodots (PNDs) is deeply modified upon thermally mediated refolding of their biological secondary structure from native metastable to stable β-sheet rich structure. This refolding leads to the appearance of a broadband optical absorption across visible region and tunable, excitation-dependent visible FL of the nanodots with a high quantum yield of ≈30%. It is shown that this intriguing biophotonic effect appears in several peptides/proteins and does not require the presence of aromatic residues. It is assumed that the origin of the phenomenon is related to proton transfer along network of reconstructed intermolecular hydrogen bonds, stabilizing the thermally induced supramolecular β-sheet structure. The biocompatible FL PNDs can be potentially applied as high-resolution bioimaging labels toward advanced biotechnology and biomedical theranostics.
KW - fluorescent peptide nanodots
KW - peptide nanophotonics
KW - peptide nanostructures
KW - refolding of peptide secondary structure
KW - visible fluorescence
UR - http://www.scopus.com/inward/record.url?scp=85059276987&partnerID=8YFLogxK
U2 - 10.1002/adom.201801400
DO - 10.1002/adom.201801400
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:85059276987
SN - 2195-1071
VL - 7
JO - Advanced Optical Materials
JF - Advanced Optical Materials
IS - 5
M1 - 1801400
ER -