Biofuel cell based on glucose oxidase and microperoxidase-11 monolayer-functionalized electrodes

Itamar Willner*, Eugenii Katz, Fernando Patolsky, Andreas F. Bückmann

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Apoglucose oxidase was reconstituted onto a pyrroloquinoline quinone and flavin adenine dinucleotide phosphate, PQQ-FAD, monolayer associated with a rough Au electrode to yield a bioelectrocatalytically active glucose oxidase, GOx. Electrically contacted PQQ-FAD/GOx monolayer was applied to the biocatalytic oxidation of glucose. A microperoxidase-11, MP-11, monolayer was assembled onto a rough Au electrode and used for the biocatalytic reduction of H2O2. Both biocatalytic electrodes, Au/PQQ-FAD/GOx and Au/MP-11, were assembled into a biofuel cell using glucose and H2O2 as the fuel substrate and the oxidizer, respectively. The biofuel cell generates an open-circuit voltage, Voc, of ca. 310 mV and a short-circuit current density, isc, of ca. 114 μA cm-2. The maximum electrical power, Pmax, extracted from the cell is 32 μW at an external optimal load of 3 kΩ. The fill factor of the biofuel cell, f = Pmax Isc-1 Voc-1, is ca. 25%.

Original languageEnglish
Pages (from-to)1817-1822
Number of pages6
JournalJournal of the Chemical Society, Perkin Transactions 2
Issue number8
StatePublished - Aug 1998
Externally publishedYes


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