Biofilm inactivation using LED systems emitting germicidal UV and antimicrobial blue light

Biofilms have been widely detected in water distribution and water storage systems posing potential risks to drinking water safety by harboring and shedding pathogens. Light-based disinfection methods, such as germicidal ultraviolet (UV) and antimicrobial blue light (aBL), could serve as non-chemical alternatives for biofilm control. This study investigated the inactivation of pure-culture Pseudomonas aeruginosa biofilms and mixed-culture biofilms using three distinct light-based disinfection methods: a low-pressure (LP) UV lamp emitting at 254 nm, a UV light emitting diode (LED) at 270 nm, and an aBL LED at 405 nm. The biofilms were developed on three commonly used materials including polycarbonate (PC), polytetrafluoroethylene (PTFE), and polyvinyl chloride (PVC), to assess the impact of surface characteristics on light-based biofilm inactivation. Our findings show that all selected devices can effectively inactivate pure-culture and mixed-culture biofilms. While both UV devices (LP UV lamp and UV LED) provided significant inactivation at lower fluences (>1 log reduction at 20 mJ/cm²), aBL LED achieved significant inactivation at higher fluences for pure culture (maximum log reduction of 3.8 ± 0.5 at > 200,000 mJ/cm²). Inactivation performance also varied with surface materials, likely attributed to different surface properties including roughness, hydrophobicity, and surface charge. This study provides important information on using light-based technologies for biofilm control and highlights the effect of surface materials on their inactivation performance.