Biocompatible nanocrystalline natural bonelike carbonated hydroxyapatite synthesized by mechanical alloying in a record minimum time

S. Lala; S. Brahmachari; P. K. Das; D. Das; T. Kar; S. K. Pradhan

doi:10.1016/j.msec.2014.06.014

Biocompatible nanocrystalline natural bonelike carbonated hydroxyapatite synthesized by mechanical alloying in a record minimum time

S. Lala, S. Brahmachari, P. K. Das, D. Das, T. Kar, S. K. Pradhan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Single phase nanocrystalline biocompatible A-type carbonated hydroxyapatite (A-cHAp) powder has been synthesized by mechanical alloying the stoichiometric mixture of CaCO₃ and CaHPO₄.2H₂O powders in open air at room temperature within 2 h of milling. The A-type carbonation in HAp is confirmed by FTIR analysis. Structural and microstructure parameters of as-milled powders are obtained from both Rietveld's powder structure refinement analysis and transmission electron microscopy. Size and lattice strain of nanocrystalline HAp particles are found to be anisotropic in nature. Mechanical alloying causes amorphization of a part of crystalline A-cHAp which is analogous to native bone mineral. Some primary bond lengths of as-milled samples are critically measured. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay test reveals high percentage of cell viability and hence confirms the biocompatibility of the sample. The overall results indicate that the processed A-cHAp has a chemical composition very close to that of biological apatite.

Original language	English
Pages (from-to)	647-656
Number of pages	10
Journal	Materials Science and Engineering C
Volume	42
DOIs	https://doi.org/10.1016/j.msec.2014.06.014
State	Published - 1 Sep 2014
Externally published	Yes

Keywords

Hydroxyapatite
MTT assay
Nanoparticles
Rietveld analysis
TEM
XRD

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10.1016/j.msec.2014.06.014

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@article{93888167720745a1a6597fcd02dce3e4,

title = "Biocompatible nanocrystalline natural bonelike carbonated hydroxyapatite synthesized by mechanical alloying in a record minimum time",

abstract = "Single phase nanocrystalline biocompatible A-type carbonated hydroxyapatite (A-cHAp) powder has been synthesized by mechanical alloying the stoichiometric mixture of CaCO3 and CaHPO4.2H2O powders in open air at room temperature within 2 h of milling. The A-type carbonation in HAp is confirmed by FTIR analysis. Structural and microstructure parameters of as-milled powders are obtained from both Rietveld's powder structure refinement analysis and transmission electron microscopy. Size and lattice strain of nanocrystalline HAp particles are found to be anisotropic in nature. Mechanical alloying causes amorphization of a part of crystalline A-cHAp which is analogous to native bone mineral. Some primary bond lengths of as-milled samples are critically measured. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay test reveals high percentage of cell viability and hence confirms the biocompatibility of the sample. The overall results indicate that the processed A-cHAp has a chemical composition very close to that of biological apatite.",

keywords = "Hydroxyapatite, MTT assay, Nanoparticles, Rietveld analysis, TEM, XRD",

author = "S. Lala and S. Brahmachari and Das, {P. K.} and D. Das and T. Kar and Pradhan, {S. K.}",

note = "Funding Information: Authors (SKP and SL) wish to thank UGC-DAE consortium for Scientific Research, Kolkata centre for providing the XRD facility and Mr. P.V. Rajesh for recording the XRD data. The University Grants Commission (UGC), India is acknowledged for granting CAS-I programme ( F. 530/5/CAS/2011/(SAP-I) ) under the thrust area “Condensed Matter Physics including Laser Applications” to the Dept. of Physics, The University of Burdwan under the financial assistance of which this work has been carried out. Authors also acknowledge Prof. B.K. Ghosh of Dept. of Chemistry, The University of Burdwan for providing the FTIR facility.-",

year = "2014",

month = sep,

day = "1",

doi = "10.1016/j.msec.2014.06.014",

language = "אנגלית",

volume = "42",

pages = "647--656",

journal = "Materials Science and Engineering C",

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TY - JOUR

T1 - Biocompatible nanocrystalline natural bonelike carbonated hydroxyapatite synthesized by mechanical alloying in a record minimum time

AU - Lala, S.

AU - Brahmachari, S.

AU - Das, P. K.

AU - Das, D.

AU - Kar, T.

AU - Pradhan, S. K.

N1 - Funding Information: Authors (SKP and SL) wish to thank UGC-DAE consortium for Scientific Research, Kolkata centre for providing the XRD facility and Mr. P.V. Rajesh for recording the XRD data. The University Grants Commission (UGC), India is acknowledged for granting CAS-I programme ( F. 530/5/CAS/2011/(SAP-I) ) under the thrust area “Condensed Matter Physics including Laser Applications” to the Dept. of Physics, The University of Burdwan under the financial assistance of which this work has been carried out. Authors also acknowledge Prof. B.K. Ghosh of Dept. of Chemistry, The University of Burdwan for providing the FTIR facility.-

PY - 2014/9/1

Y1 - 2014/9/1

N2 - Single phase nanocrystalline biocompatible A-type carbonated hydroxyapatite (A-cHAp) powder has been synthesized by mechanical alloying the stoichiometric mixture of CaCO3 and CaHPO4.2H2O powders in open air at room temperature within 2 h of milling. The A-type carbonation in HAp is confirmed by FTIR analysis. Structural and microstructure parameters of as-milled powders are obtained from both Rietveld's powder structure refinement analysis and transmission electron microscopy. Size and lattice strain of nanocrystalline HAp particles are found to be anisotropic in nature. Mechanical alloying causes amorphization of a part of crystalline A-cHAp which is analogous to native bone mineral. Some primary bond lengths of as-milled samples are critically measured. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay test reveals high percentage of cell viability and hence confirms the biocompatibility of the sample. The overall results indicate that the processed A-cHAp has a chemical composition very close to that of biological apatite.

AB - Single phase nanocrystalline biocompatible A-type carbonated hydroxyapatite (A-cHAp) powder has been synthesized by mechanical alloying the stoichiometric mixture of CaCO3 and CaHPO4.2H2O powders in open air at room temperature within 2 h of milling. The A-type carbonation in HAp is confirmed by FTIR analysis. Structural and microstructure parameters of as-milled powders are obtained from both Rietveld's powder structure refinement analysis and transmission electron microscopy. Size and lattice strain of nanocrystalline HAp particles are found to be anisotropic in nature. Mechanical alloying causes amorphization of a part of crystalline A-cHAp which is analogous to native bone mineral. Some primary bond lengths of as-milled samples are critically measured. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay test reveals high percentage of cell viability and hence confirms the biocompatibility of the sample. The overall results indicate that the processed A-cHAp has a chemical composition very close to that of biological apatite.

KW - Hydroxyapatite

KW - MTT assay

KW - Nanoparticles

KW - Rietveld analysis

KW - TEM

KW - XRD

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C2 - 25063165

AN - SCOPUS:84903647926

SN - 0928-4931

VL - 42

SP - 647

EP - 656

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

ER -

Biocompatible nanocrystalline natural bonelike carbonated hydroxyapatite synthesized by mechanical alloying in a record minimum time

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Keywords

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