TY - JOUR
T1 - Biocompatible Hybrid Organic/Inorganic Microhydrogels Promote Bacterial Adherence and Eradication in Vitro and in Vivo
AU - Schnaider, Lee
AU - Toprakcioglu, Zenon
AU - Ezra, Assaf
AU - Liu, Xizhou
AU - Bychenko, Darya
AU - Levin, Aviad
AU - Gazit, Ehud
AU - Knowles, Tuomas P.J.
N1 - Publisher Copyright:
© 2020 American Chemical Society.
PY - 2020/3/11
Y1 - 2020/3/11
N2 - Self-assembling peptides and proteins have the potential to serve as multifunctional building blocks for the generation of versatile materials for a wide range of biomedical applications. In particular, supramolecular hydrogels comprised of self-assembled protein nanofibrils, have been used in contexts ranging from tissue engineering to drug delivery. Due to the rapid emergence of multidrug resistant bacteria, development of biomaterials with intrinsic antimicrobial properties has been continuously increasing. Here, we describe hybrid organic/inorganic nanofibrillar silk microgels decorated with silver nanoparticles that display potent antimicrobial activity in vitro and in vivo and are able to adhere bacterial cells to their surfaces while subsequently eradicating them, through a two-step mechanism of action. Importantly, in contrast to treatments involving conventional silver, these silk-silver microgels are nonhemolytic and noncytotoxic toward mammalian cell lines. Finally, we show that these hybrid microgels display substantial efficacy as topical antimicrobial agents in a murine model of surgical site infections.
AB - Self-assembling peptides and proteins have the potential to serve as multifunctional building blocks for the generation of versatile materials for a wide range of biomedical applications. In particular, supramolecular hydrogels comprised of self-assembled protein nanofibrils, have been used in contexts ranging from tissue engineering to drug delivery. Due to the rapid emergence of multidrug resistant bacteria, development of biomaterials with intrinsic antimicrobial properties has been continuously increasing. Here, we describe hybrid organic/inorganic nanofibrillar silk microgels decorated with silver nanoparticles that display potent antimicrobial activity in vitro and in vivo and are able to adhere bacterial cells to their surfaces while subsequently eradicating them, through a two-step mechanism of action. Importantly, in contrast to treatments involving conventional silver, these silk-silver microgels are nonhemolytic and noncytotoxic toward mammalian cell lines. Finally, we show that these hybrid microgels display substantial efficacy as topical antimicrobial agents in a murine model of surgical site infections.
KW - Antibacterial materials
KW - Biomaterials
KW - Microhydrogels
KW - Organic/inorganic interfaces
KW - Regenerated silk fibroin
KW - Self-assembly
UR - http://www.scopus.com/inward/record.url?scp=85080965850&partnerID=8YFLogxK
U2 - 10.1021/acs.nanolett.9b04290
DO - 10.1021/acs.nanolett.9b04290
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 32040332
AN - SCOPUS:85080965850
SN - 1530-6984
VL - 20
SP - 1590
EP - 1597
JO - Nano Letters
JF - Nano Letters
IS - 3
ER -