Biocompatible agarose-chitosan coated silver nanoparticle composite for soft tissue engineering applications

Nupur Kumar, Dayananda Desagani, Girish Chandran, Narendra Nath Ghosh, Ganesh Karthikeyan, Sachin Waigaonkar, Anasuya Ganguly*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


With increasing gap in the demand and supply of vital organs for transplantation there is a pressing need to bridge the gap with substitutes. One way to make substitutes is by tissue engineering which involves combining several types of synthetic or biomaterials, cells and growth factors cross-linked together to synthesize a functional scaffold for repair or replacement of non-functional organs. Nanoparticle based composites are gaining importance in tissue engineering due to their ability to enhance cell attachment and proliferation. The current study focuses on synthesizing agarose composites embedded with chitosan-coated silver nanoparticles using glutaraldehyde as the cross-linker. The synthesis of chitosan coated silver nanoparticles within the scaffold was confirmed with UV-visible spectroscopy. Physical and chemical characterization of the synthesized nanoparticles were done by XRD, FTIR, TGA and SEM. DMA showed higher mechanical strength of the scaffolds. The scaffolds showed degradation of ∼37% within a span of four weeks. The higher physical support provided by the synthesized scaffolds was shown by in-vitro cell viability assay. Broad spectrum anti-bacterial activity and superior hemocompatibility further showed the advantage it offered for growing cells. Thus a biopolymer based nanocomposite was synthesized, with intended widespread use as scaffold for engineering of soft tissues due to its enhanced biocompatibility and greater surface area for cell growth.

Original languageEnglish
Pages (from-to)637-649
Number of pages13
JournalArtificial Cells, Nanomedicine and Biotechnology
Issue number3
StatePublished - 3 Apr 2018
Externally publishedYes


  • Agarose
  • chitosan
  • scaffolds
  • silver nanoparticles
  • tissue engineering


Dive into the research topics of 'Biocompatible agarose-chitosan coated silver nanoparticle composite for soft tissue engineering applications'. Together they form a unique fingerprint.

Cite this