@article{448d94ce9bfa4ae1b7335f2ce72bc668,
title = "Biochemical evidence that acetylcholine release from cholinergic nerve terminals is mostly vesicular",
abstract = "The nature of the intraterminal compartments from which acetylcholine (ACh) is released following presynaptic stimulation was investigated. This was pursued by examining the effects of the anticholinergic drug 2-(4-phenylpiperidino)cyclohexanol (AH5183) on the release of newly synthesized [3H]ACh and of endogenous ACh from purified cholinergic nerve terminals (synaptosomes) which were isolated from the electric organs of Torpedo. Preincubation of the synaptosomes, with AH5183 (1-10 μM), does not affect either the intraterminal synthesis of [3H]ACh or the uptake of its precursors, but results in a marked inhibition (85%) of the release of the newly synthesized [3H]ACh. However, when AH5183 is added following the accumulation of [3H]ACh in the nerve terminals, it does not affect [3H]ACh release. AH5183 also has no effect on the release of preformed endogenous ACh. These findings, together with the previous in vitro demonstrations that AH5183 is a potent inhibitor of ACh uptake into isolated cholinergic vesicles, suggest that most of the synaptosomal ACh is secreted by a vesicular mechanism.",
keywords = "AH5183, Acetylcholine, Cholinergic synoptic vesicle, Synaptosome, Torpedo",
author = "Michaelson, {Daniel M.} and M. Burstein",
note = "Funding Information: We thank Dr D.E. Bays of Glaxo Group Re-search Ltd for the gift of AH5183. This work was supportedi n part by the Familial Dysautonomia Foundation, The US-Israel Binational Science Foundation (BSF Grant no. 2410)a nd the Fund for Basic Researchs ponsoredb y the Israel Academy of Sciencesa nd Humanities.",
year = "1985",
month = sep,
day = "2",
doi = "10.1016/0014-5793(85)80408-7",
language = "אנגלית",
volume = "188",
pages = "389--393",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "John Wiley and Sons Inc.",
number = "2",
}