Biochemical characterization of the type C retrovirus associated with lymphoproliferative disease of turkeys

A. Yaniv, A. Gazit, M. Ianconescu, K. Perk, B. Aizenberg, A. Zimber

Research output: Contribution to journalArticlepeer-review

Abstract

Turkeys inoculated with spleen extracts from lymphoproliferative disease (LPD)-affected birds developed viremia, followed by typical LPD lesions. Electron microscopy and biochemical characterization established that the virus present in the blood of infected turkeys is a type C retrovirus. The viral particles possess a buoyant density of 1.17 g/ml in sucrose gradients; they contain high-molecular weight RNA and an RNA-instructed DNA polymerase with efficient exogenous and endogenous activity. The LPD virus polymerase is preferentially activated by magnesium ions. Cross nucleic acid hybridization assays revealed no sequence homology between the viral genome of LPD and avian myeloblastosis virus or reticuloendotheliosis virus, thus indicating that the LPD virus belongs to a distinct group unrelated to the avian leukosis-sarcoma virus complex or to the reticuloendotheliosis virus group.

Original languageEnglish
Pages (from-to)351-357
Number of pages7
JournalUnknown Journal
Volume30
Issue number1
DOIs
StatePublished - 1979

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