Bio-H2 was produced by Enterobacter sp., using de-oiled cake (DOC) as nitrogen source and was subsequently converted to electricity by a hybrid platform of proton exchange membrane fuel cell (PEM-FC) and photo voltaic (PV) cells. Taguchi design was used to maximize H2 production on using different DOC concentrations, pH, pre-treatment time and agitation speeds. Highest H2 production of 3.42 ± 0.26 L L−1 was observed at pH 6.0, 50 g L−1 DOC concentration, 20 min pre-treatment time and 200 rpm agitation speed, respectively. In continuous process maximum rate of H2 production of 854 ± 20 mL L−1 h−1 (molasses), 580 ± 22 mL L−1 h−1 (glucose) and 640 ± 22 mL L−1 h−1 (starchy wastewater), respectively was observed at 0.2 h−1 dilution rate. The hybrid system showed maximum current (I), voltage (V) and power (P) of 0.035 mA, 11 V and 385 mW, respectively.
- Continuous hydrogen production
- De-oiled cake
- Fuel cell
- Taguchi design