Bio-degradation of a resorbable collagen membrane (Bio-Gide ®) applied in a double-layer technique in rats

Avital Kozlovsky*, Guy Aboodi, Ofer Moses, Haim Tal, Zvi Artzi, Miron Weinreb, Carlos E. Nemcovsky

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: The aim of this study was to evaluate histologically the bio-degradation of two layers of Bio-Gide® (BG) membrane, as compared with that of a single layer. Material and methods: Two circular calvarial bony defects, 5 mm in diameter, were made in 24 Wistar rats. BG membrane, labeled with biotin, was cut into 5-mm-diameter disks, and placed in defects either as a mono-layer membrane (MLM) or as a double-layer membrane (DLM). Rats were sacrificed after 4 or 9 weeks and histology was performed. Membranes were stained with horseradish peroxidase-conjugated streptavidin and aminoethyl carbazole as a substrate for detection of biotinylated collagen. The area of collagen and thickness of the residual membranes were measured by image analysis software. Statistical analysis was performed using the non-parametric Wilcoxon's signed-ranks test. Results: At 4-week collagen area per measurement window within the DLM sites (0.09±0.05 mm2) was significantly greater (P<0.01) than that in the MLM sites (0.047±0.034 mm 2). At 9 weeks, the collagen area was also greater in the DLM sites (0.037±0.026 mm2) compared with that of the MLM sites (0.025±0.016 mm2); however, this difference did not reach statistical significance. The rate of membrane degradation, calculated as percent membrane lost compared with baseline, was similar for the DLM and MLM at both time points (∼60% at 4 weeks and ∼80% at 9 weeks). In addition, the residual DLM thickness at 4 weeks (475.5±73.77 μm) was significantly (P<0.01) greater than that of MLM (262.38±48.01 μm). At 9 weeks, membrane thickness was also greater in the DLM sites (318.22±70.45 μm) compared with that of the MLM sites (183.32±26.72 μm); however, this difference did not reach statistical significance. The reduction in thickness between 4 and 9 weeks was 30% for MLM and 33% for DLM. Discussion: The use of a double layer of BG membrane results in a barrier of increased collagen area and thickness, compared with application of a single layer.

Original languageEnglish
Pages (from-to)1116-1123
Number of pages8
JournalClinical Oral Implants Research
Volume20
Issue number10
DOIs
StatePublished - Oct 2009

Keywords

  • Barrier membranes
  • Collagen degradation
  • Guided bone regeneration

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