Bimodal X-ray and Infrared Imaging of an Organometallic Derivative of Praziquantel in Schistosoma mansoni

Sylvain Clède, Noemi Cowan, François Lambert, Hélène C. Bertrand, Riccardo Rubbiani, Malay Patra, Jeannine Hess, Christophe Sandt, Nicolas Trcera, Gilles Gasser*, Jennifer Keiser, Clotilde Policar

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

An organometallic derivative of praziquantel was studied directly in worms by using inductively coupled plasma-mass spectrometry (ICP-MS) for quantification and synchrotron-based imaging. X-ray fluorescence (XRF) and IR absorption spectromicroscopy were used for the first time in combination to directly locate this organometallic drug candidate in schistosomes. The detection of both CO (IR) and Cr (XRF) signatures proved that the Cr(CO)3 core remained intact in the worms. Images showed a preferential accumulation at the worm's tegument, consistent with a possible targeting of the calcium channel but not excluding other biological targets inside the worm. Imaginative imaging: Two synchrotron-based techniques - X-ray fluorescence and IR absorption spectroscopy - were used in combination for the first time to directly locate an organometallic drug candidate in schistosomes. This represents a novel approach to examine mechanisms of actions for organometallic compounds and might lead to the discovery of new drug candidates.

Original languageEnglish
Pages (from-to)1004-1007
Number of pages4
JournalChemBioChem
Volume17
Issue number11
DOIs
StatePublished - 2 Jun 2016
Externally publishedYes

Keywords

  • IR absorption spectroscopy
  • X-ray fluorescence
  • chromium
  • organometallic chemistry
  • schistosomes

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