TY - JOUR
T1 - Behavioral classification of excitatory amino acid receptors in mouse spinal cord
AU - Urca, Gideon
AU - Raigorodsky, Gladys
N1 - Funding Information:
This work was supported by the Israel Institure for Psychobiology; The Charles, E. Smith Foundation grant to G.U.
PY - 1988/8/24
Y1 - 1988/8/24
N2 - Intrathecal injections of excitatory amino acid (EAA) agonists to the spinal cord of mice produces behavioral activation manifest as biting and scratching of the hindquarters. The dose-response relationship of EAA (N-methyl-D-aspartate (NMDA), kainate, quisqualate and glutamate)-induced activation revealed qualitative and quantitative differences in their pattern of action, suggesting that these agonists act at distinct receptors. Evaluation of the blockade of EAA-induced bites by a series of antagonists: DL-2-amino-5-phosphonovalerate (APV), γ-D-glutamyl glycine (DGG), kynurenate and glutamylaminomethylsulphonate (GAMS), indicated that selective activation of the NMDA, quisqualate and kainate receptors can be demonstrated using this behavior. The NMDA receptors could be subdivided on the basis of different sensitivity to kynurenate and APV. Antagonist-resistant components of both kainate and quisqualate action were also shown. Thus, the biting behavior induced by the administration of intrathecal EAA agonists can be used as a relatively selective behavioral tool for assessing the pharmacological profile of action of excitatory amino acid agonists and antagonists in the spinal cord.
AB - Intrathecal injections of excitatory amino acid (EAA) agonists to the spinal cord of mice produces behavioral activation manifest as biting and scratching of the hindquarters. The dose-response relationship of EAA (N-methyl-D-aspartate (NMDA), kainate, quisqualate and glutamate)-induced activation revealed qualitative and quantitative differences in their pattern of action, suggesting that these agonists act at distinct receptors. Evaluation of the blockade of EAA-induced bites by a series of antagonists: DL-2-amino-5-phosphonovalerate (APV), γ-D-glutamyl glycine (DGG), kynurenate and glutamylaminomethylsulphonate (GAMS), indicated that selective activation of the NMDA, quisqualate and kainate receptors can be demonstrated using this behavior. The NMDA receptors could be subdivided on the basis of different sensitivity to kynurenate and APV. Antagonist-resistant components of both kainate and quisqualate action were also shown. Thus, the biting behavior induced by the administration of intrathecal EAA agonists can be used as a relatively selective behavioral tool for assessing the pharmacological profile of action of excitatory amino acid agonists and antagonists in the spinal cord.
KW - Excitatory amino acid receptors
KW - Excitatory amino acids
KW - Spinal cord
UR - http://www.scopus.com/inward/record.url?scp=0023726747&partnerID=8YFLogxK
U2 - 10.1016/0014-2999(88)90608-5
DO - 10.1016/0014-2999(88)90608-5
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AN - SCOPUS:0023726747
SN - 0014-2999
VL - 153
SP - 211
EP - 220
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 2-3
ER -