TY - JOUR
T1 - Behavioral and neurochemical effects of intraventricular AF64A administration in rats
AU - Jarrard, Leonard E.
AU - Kant, G. Jean
AU - Meyerhoff, James L.
AU - Levy, Aharon
N1 - Funding Information:
In the place task, the rat learns to choose four out of eight similar arms where all arms remain in the same spatial location and the same arms are baited from trial to trial. In the cue task, textured floor inserts in the eight arms are moved in a random order from trial to trial and the rat is rewarded for ~The views of the author(s) do not purport to reflect the position of the Department of the Army or the Department of Defense (para 4-3, AR 360-5). 2In conducting the research described in this report, the investigator(s) adhere to the "Guide for the Care and Use of Laboratory Animals," as promulgated by the Committee on Care and Use of Laboratory Animals of the Institute of Laboratory Animal Resources, National Research Council. :~Supported in part by a grant (BN, 80-05735) from the National Science Foundation to L.E.J. 4Visiting scientist from the Israel Institute for Biological Research, Ness Ziona, Israel. Recipient of a Senior National Research Council Fellowship.
PY - 1984/8
Y1 - 1984/8
N2 - Ethylcholine aziridinium ion solution (AF64A), a putative specific cholinergic neurotoxin, was injected bilaterally into the lateral ventricles of rats. Following administration of 3 or 6 nmoles of AF64A, drinking and eating were depressed but returned to normal levels after several days; increased activity in the 6 nmole group persisted throughout the 21 days of observations. Performance on complex place and cue tasks indicated that injected animals were impaired in reference memory only on the place task, but working memory was impaired on both tasks. Neurochemical measurements in a separate group of animals one week after AF64A injections found large depletions of acetylcholine in hippocampus and corpus striatum, but not depletions of norepinephrine (hippocampus) or dopamine (striatum). Histological examination of the injection site revealed extensive damage to the fimbria-fornix similar to that seen after electrolytic lesions. Since the behavioral and neurochemical changes are similar to those previously found following fimbria-fornix lesions, it is concluded that the present results are possibly due to non-specific lesion effects of the neurotoxin rather than a specific effect on cholinergic systems.
AB - Ethylcholine aziridinium ion solution (AF64A), a putative specific cholinergic neurotoxin, was injected bilaterally into the lateral ventricles of rats. Following administration of 3 or 6 nmoles of AF64A, drinking and eating were depressed but returned to normal levels after several days; increased activity in the 6 nmole group persisted throughout the 21 days of observations. Performance on complex place and cue tasks indicated that injected animals were impaired in reference memory only on the place task, but working memory was impaired on both tasks. Neurochemical measurements in a separate group of animals one week after AF64A injections found large depletions of acetylcholine in hippocampus and corpus striatum, but not depletions of norepinephrine (hippocampus) or dopamine (striatum). Histological examination of the injection site revealed extensive damage to the fimbria-fornix similar to that seen after electrolytic lesions. Since the behavioral and neurochemical changes are similar to those previously found following fimbria-fornix lesions, it is concluded that the present results are possibly due to non-specific lesion effects of the neurotoxin rather than a specific effect on cholinergic systems.
KW - Acetylcholine depletion
KW - AF64A
KW - Ethylcholine aziridinium ion
KW - Fimbria-fornix lesion
KW - Memory deficits
KW - Radial arm maze
UR - http://www.scopus.com/inward/record.url?scp=0021207372&partnerID=8YFLogxK
U2 - 10.1016/0091-3057(84)90226-0
DO - 10.1016/0091-3057(84)90226-0
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C2 - 6548313
AN - SCOPUS:0021207372
VL - 21
SP - 273
EP - 280
JO - Pharmacology Biochemistry and Behavior
JF - Pharmacology Biochemistry and Behavior
SN - 0091-3057
IS - 2
ER -