@article{b6e207ec646044b990450741f5ec1e02,
title = "Bcl-x(L) inhibits different apoptotic pathways in rat PC12 cells",
abstract = "Bcl-x(L), a member of the bcl-2 family of proteins is required for the survival of neurons early in development. To study the mechanism of action of Bcl-x(L) in a neuronal context, we generated rat PC12 cells overexpressing Bcl-x(L) and examined their susceptibility to apoptotic stimuli that induce apoptosis through different pathways involving trophic-factor deprivation, staurosporine, tumor necrosis factor alpha or cisplatin. Overexpression of Bcl-x(L) in both naive and neuronal PC12 cells inhibited apoptosis induced by the different pathways. However, the extent of this protective effect varied, suggesting that the contribution of the Bcl-x(L)-controlled step to apoptosis differs in the different pathways. Our findings also showed that TNFα- induced activation of caspase-3 is inhibited by overexpression of Bcl-x(L), suggesting that Bcl-x(L) acts upstream of caspase activation.",
keywords = "Apoptosis, Bcl-x(L), Caspases, PC12 cells, TNFα",
author = "Liora Lindenboim and Ronit Haviv and Reuven Stein",
note = "Funding Information: We thank Dr. Craig Thompson for the plasmid containing Bcl-x L cDNA and Ms. Shirley Smith for excellent editorial assistance. This work was supported by the Israel Science Foundation and the German-Israeli Foundation.",
year = "1998",
month = aug,
day = "28",
doi = "10.1016/S0304-3940(98)00602-8",
language = "אנגלית",
volume = "253",
pages = "37--40",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1",
}
