Baseline oxysterols and other markers of oxidative stress, inflammation and malnutrition in the vitamin E and intima media thickness progression in end-stage renal disease (VIPER) cohort

Mona Boaz, Luigi Iuliano, Jonathan Himmelfarb, Zipora Matas, Fausta Micheletta, Ellen McMonagle, Victoria Friedman, Silvia Natoli, Gabriella Gvirtz, Alexander Biro, Shmuel Smetana, Gideon Sabo, Uzi Gafter, Talia Weinstein

Research output: Contribution to journalArticlepeer-review

Abstract

Background and Objectives: Oxysterols are markers of oxidative stress, levels of which have not yet been reported in hemodialysis (HD) patients. This study was designed to compare levels of the oxysterols 7-ketocholesterol (7KC) and 7β-hydroxycholesterol (7βOH) between a cohort of HD patients and healthy controls. Methods: This nested cross-sectional study reflects baseline (preintervention) values for markers of oxidative stress, inflammation and nutrition status in the 160-member vitamin E and carotid intima media thickness progression in end-stage renal disease (VIPER) cohort (age 64.1 ± 8.8, 33.5% female). Age- and sex-matched healthy volunteers served as controls. Plasma oxysterols 7KC and 7βOH were determined by isotope dilution gas chromatography/mass spectrometry. Results: Despite higher plasma α-tocopherol levels in HD patients than controls (36.0 ± 9.3 vs. 31.8 ± 8.4 μmol/l, p = 0.007), 7KC levels (9.8 ± 6.9 vs. 5.9 ± 2.8 nmol/mmol cholesterol, p < 0.0001) and 7βOH levels (8.7 ± 4.3 vs. 2.7 ± 1.6 nmol/ mmol cholesterol, p < 0.0001) were higher in HD patients. The oxysterol 7βOH was significantly, inversely associated with prealbumin (r = -0.18, p = 0.03), though neither oxysterol was significantly associated with any other marker of oxidative stress, inflammation or nutrition status and did not discriminate for CVD in HD patients. Conclusions: Elevated levels of the oxysterols 7KC and 7βOH indicate that HD patients are in a state of oxidative stress compared to healthy controls. However, oxysterols 7KC and 7βOH did not appear to contribute additional information about oxidative stress among HD patients.

Original languageEnglish
Pages (from-to)c111-c119
JournalNephron - Clinical Practice
Volume100
Issue number4
DOIs
StatePublished - Aug 2005
Externally publishedYes

Keywords

  • Hemodialysis
  • Oxidative stress
  • Oxysterols

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