@article{a707b93267cf468696f53036a743a6d8,
title = "Bacteriophage strain typing by rapid single molecule analysis",
abstract = "Rapid characterization of unknown biological samples is under the focus of many current studies. Here we report a method for screening of biological samples by optical mapping of their DNA. We use a novel, one-step chemo-enzymatic reaction to covalently bind fluorophores to DNA at the four-base recognition sites of a DNA methyltransferase. Due to the diffraction limit of light, the dense distribution of labels results in a continuous fluorescent signal along the DNA. The amplitude modulations (AM) of the fluorescence intensity along the stretched DNA molecules exhibit a unique molecular fingerprint that can be used for identification. We show that this labelling scheme is highly informative, allowing accurate genotyping. We demonstrate the method by labelling the genomes of λ and T7 bacteriophages, resulting in a consistent, unique AM profile for each genome. These profiles are also successfully used for identification of the phages from a background phage library. Our method may provide a facile route for screening and typing of various organisms and has potential applications in metagenomics studies of various ecosystems.",
author = "Assaf Grunwald and Moran Dahan and Anna Giesbertz and Adam Nilsson and Nyberg, {Lena K.} and Elmar Weinhold and Tobias Ambj{\"o}rnsson and Fredrik Westerlund and Yuval Ebenstein",
note = "Publisher Copyright: {\textcopyright} The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.",
year = "2015",
month = oct,
day = "15",
doi = "10.1093/nar/gkv563",
language = "אנגלית",
volume = "43",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "18",
}