Bacterial growth on Mueller Hinton medium sterilized by γ-radiation

Eliahu Eisenberg, Bianca Bogokowsky, Gideon Altmann

Research output: Contribution to journalArticlepeer-review


The possibility of radiosterilization of culture media for the cultivation of bacteria was investigated. Mueller Hinton agar, a medium widely used for the propagation of some fastidious pathogenic bacteria, was sterilized with 1.0 and 1.5 Mrad doses of γ-radiation. Bacteria belonging to seven different species grew as well on the radiosterilized medium as on medium sterilized by heat in a conventional way. Reduction in colony size was observed on the radiosterilized medium with Staphylococcus aureus and Shigella boydii. Neisseria meningitidis, the most fastidious bacterium tested, did not grow at all. The addition of small amounts of catalase corrected the deleterious radiation effect and all bacteria tested could be successfully grown on irradiated Mueller Hinton agar supplemented with up to 11 Keil units catalase per liter medium.

Original languageEnglish
Pages (from-to)891-893
Number of pages3
JournalApplied Radiation and Isotopes
Issue number12
StatePublished - Dec 1981
Externally publishedYes


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