Azide, an inhibitor of ATPase, and a specific inhibitor of protein export was used in order to select for protein secretion mutants in Acinetobacter calcoaceticus A2. Two such mutants were isolated that were azide-resistant and defective in the general protein transport system. The mutation also conferred additional phenotypic changes, including an inability to grow on minimal media or at 40°C. The existence of protein secretion mutants with a selectable phenotype may be useful for the genetic study of protein export.
|Number of pages||4|
|Journal||FEMS Microbiology Letters|
|State||Published - 15 Feb 1994|
- Acinetobacter calcoaceticus
- Protein export
- Protein secretion