Azide-resistant mutants in Acinetobacter calcoaceticus A2 are defective in protein secretion

Adi Elkeles, Eugene Rosenberg, Eliora Z. Ron*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Azide, an inhibitor of ATPase, and a specific inhibitor of protein export was used in order to select for protein secretion mutants in Acinetobacter calcoaceticus A2. Two such mutants were isolated that were azide-resistant and defective in the general protein transport system. The mutation also conferred additional phenotypic changes, including an inability to grow on minimal media or at 40°C. The existence of protein secretion mutants with a selectable phenotype may be useful for the genetic study of protein export.

Original languageEnglish
Pages (from-to)221-224
Number of pages4
JournalFEMS Microbiology Letters
Volume116
Issue number2
DOIs
StatePublished - 15 Feb 1994

Keywords

  • Acinetobacter calcoaceticus
  • Azide
  • Protein export
  • Protein secretion

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