TY - JOUR
T1 - Autoxidation and mutagenicity of sodium bisulfite
AU - Pagano, Dennis A.
AU - Zeiger, Errol
AU - Stark, Avishay Abraham
PY - 1990/1
Y1 - 1990/1
N2 - An inverse correlation exists between the autoxidation of bisulfite and its mutagenicity in Salmonella. Temperature, pH, and the addition of mannitol, ethanol, or Oxoid broth affect both autoxidation and mutagenicity. A decrease in autoxidation resulted in an increase in the half-life of the parent compound, bisulfite, and its availability for uptake by the cells, leading to increased mutagenesis. The autoxidation of bisulfite is known to produce both sulfur- and oxygen-centered free radicals. The lack of mutagenicity of ammonium persulfate and peroxymonosulfate, which generate the radicals SO4- and SO5-, respectively, argues against the involvement of these oxygen-centered radicals in bisulfite mutagenesis. Inhibition of mutagenesis by the radical spin-trapping agent, DMPO, is consistent with the hypothesis that the sulfur-centered radical, SO3-, plays an important role in bisulfite mutagenesis. The mechanism of bisulfite mutagenesis suggested in this study may have relevance to other known effects attributed to bisulfite, i.e., co-carcinogenesis and immune hypersensitivity.
AB - An inverse correlation exists between the autoxidation of bisulfite and its mutagenicity in Salmonella. Temperature, pH, and the addition of mannitol, ethanol, or Oxoid broth affect both autoxidation and mutagenicity. A decrease in autoxidation resulted in an increase in the half-life of the parent compound, bisulfite, and its availability for uptake by the cells, leading to increased mutagenesis. The autoxidation of bisulfite is known to produce both sulfur- and oxygen-centered free radicals. The lack of mutagenicity of ammonium persulfate and peroxymonosulfate, which generate the radicals SO4- and SO5-, respectively, argues against the involvement of these oxygen-centered radicals in bisulfite mutagenesis. Inhibition of mutagenesis by the radical spin-trapping agent, DMPO, is consistent with the hypothesis that the sulfur-centered radical, SO3-, plays an important role in bisulfite mutagenesis. The mechanism of bisulfite mutagenesis suggested in this study may have relevance to other known effects attributed to bisulfite, i.e., co-carcinogenesis and immune hypersensitivity.
KW - Autoxidation and mutagenesis
KW - Bisulphite, sodium
KW - Free radicals
KW - Salmonella
KW - pH
UR - http://www.scopus.com/inward/record.url?scp=0025192653&partnerID=8YFLogxK
U2 - 10.1016/0027-5107(90)90016-W
DO - 10.1016/0027-5107(90)90016-W
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AN - SCOPUS:0025192653
SN - 0027-5107
VL - 228
SP - 89
EP - 96
JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
IS - 1
ER -