Autologous mixed lymphocyte reaction in man: Further characterization of responding cells

Abraham Klajman, Ilana Drucker, Yosef Manor

Research output: Contribution to journalArticlepeer-review

Abstract

Experiments were performed to determine the type of cells undergoing thymidine incorporation in 7-day autologous mixed lymphocyte reactions (AMLR). Peripheral blood mononuclear cells (PBM) were separated into B cells, T cells, B + Null cells and T + Null cell-enriched populations. Cells were cultured in various combinations. Monocytes were removed to determine their influence on AMLR. The main thymidine-incorporating cells in cultures were shown to be Null cells and to a lesser extent T cells. Monocytes were found to have a more pronounced suppressor effect on stimulation of T cells by non-T cell populations in younger individuals than in the elderly. Whether Null cells undergo a spontaneous blastogenesis in culture or are stimulated by T or B cells, could not be answered by the present investigation.

Original languageEnglish
Pages (from-to)13-19
Number of pages7
JournalImmunology Letters
Volume6
Issue number1
DOIs
StatePublished - Jan 1983
Externally publishedYes

Keywords

  • Null cells
  • autologous mixed lymphocyte reaction

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