Augmentation of the expression of proangiogenic genes in cardiomyocytes with low dose laser irradiation In vitro

Ashwani Khanna, Latha Raja Shankar, Michael H. Keelan, Ran Kornowski, Martin Leon, Jeffrey Moses, Nicholas Kipshidze

Research output: Contribution to journalArticlepeer-review


Background and Objective. Several reports suggest that low power red laser light (LPRLL) is capable of affecting cellular processes in the absence of significant thermal effect. The objective of the present study was to determine the effect of LPRLL on proliferation of fetal cardiomyocytes in vitro and on the expression of proangiogenic genes, transforming growth factor-β (TGF-β), and vascular endothelial growth factor (VEGF). Study Design/Materials and Methods. All cell cultures were irradiated with single-dose LPRLL using a He-Ne continuous wave laser (632 nm) with different doses. The effect of LPRLL on new DNA synthesis was studied by 3H thymidine-incorporation assay. VEGF and TGF-β expression by cardiomyocytes was studied by reverse transcription-polymerase chain reaction (RT-PCR). Results. We observed that a dose-dependent increase in cardiomyocytes proliferation can be obtained with LPRLL and that there is a significant increase in VEGF and TGF-β mRNA expression by cardiomyocytes. Conclusions. These data may have significant importance leading to the establishment of new methods for myocardial photoangiogenesis and photoregeneration as well as in vitro proliferation of cardiac myocytes.

Original languageEnglish
Pages (from-to)265-269
Number of pages5
JournalCardiovascular Radiation Medicine
Issue number3
StatePublished - Jul 1999
Externally publishedYes


  • Angiogenesis, Regeneration
  • Coronary artery disease
  • Lasers


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