TY - JOUR
T1 - Attempted application of bioengineered/biosynthetic supporting matrices with phosphatidylinositol-trisphosphate-enhancing substances to organ culture of human primordial follicles
AU - Lerer-Serfaty, Galit
AU - Samara, Nivin
AU - Fisch, Benjamin
AU - Shachar, Michal
AU - Kossover, Olga
AU - Seliktar, Dror
AU - Ben-Haroush, Avi
AU - Abir, Ronit
N1 - Funding Information:
Disclosure summary RA and BF received grant support from Leo Minz Fund, Tel Aviv University and from the Israel Cancer Association, GL-S, NS, MS, OK, DS, SC and AB-H have nothing to declare.
PY - 2013/10
Y1 - 2013/10
N2 - Purpose: To improve human primordial follicle culture. Methods: Thin or thick ovarian slices were cultured on alginate scaffolds or in PEG-fibrinogen hydrogels with or without bpV (pic), which prevents the conversion of phosphatidylinositol-trisphosphate (PIP3) to phosphatidylinositol-bisphosphate (PIP2) or 740Y-P which converts PIP2 to PIP3. Follicular growth was evaluated by follicular counts, Ki67 immunohistochemistry, and 17β-estradiol (E2) levels. Results: BpV (pic) had a destructive effect on cultured follicles. Thawed-uncultured samples had more primordial follicles than samples cultured in basic medium and fewer developing follicles than samples cultured in PEG-fibrinogen hydrogels with 740Y-P. There were more atretic follicles in samples cultured on alginate scaffolds than in PEG-fibrinogen hydrogels, and in samples cultured in PEG-fibrinogen hydrogels with 740Y-P than in PEG-fibrinogen hydrogels with basic medium. Ki67 staining was higher in PEG-fibrinogen hydrogels than on alginate scaffolds. E2 levels were higher in thick than in thin slices. Conclusions: PEG-fibrinogen hydrogels appear to have an advantage over alginate scaffolds for culturing human primordial follicles. Folliculogenesis is not increased in the presence of substances that enhance PIP3 production or with thin rather than thick sectioning.
AB - Purpose: To improve human primordial follicle culture. Methods: Thin or thick ovarian slices were cultured on alginate scaffolds or in PEG-fibrinogen hydrogels with or without bpV (pic), which prevents the conversion of phosphatidylinositol-trisphosphate (PIP3) to phosphatidylinositol-bisphosphate (PIP2) or 740Y-P which converts PIP2 to PIP3. Follicular growth was evaluated by follicular counts, Ki67 immunohistochemistry, and 17β-estradiol (E2) levels. Results: BpV (pic) had a destructive effect on cultured follicles. Thawed-uncultured samples had more primordial follicles than samples cultured in basic medium and fewer developing follicles than samples cultured in PEG-fibrinogen hydrogels with 740Y-P. There were more atretic follicles in samples cultured on alginate scaffolds than in PEG-fibrinogen hydrogels, and in samples cultured in PEG-fibrinogen hydrogels with 740Y-P than in PEG-fibrinogen hydrogels with basic medium. Ki67 staining was higher in PEG-fibrinogen hydrogels than on alginate scaffolds. E2 levels were higher in thick than in thin slices. Conclusions: PEG-fibrinogen hydrogels appear to have an advantage over alginate scaffolds for culturing human primordial follicles. Folliculogenesis is not increased in the presence of substances that enhance PIP3 production or with thin rather than thick sectioning.
KW - Alginate scaffolds
KW - Human primordial follicles
KW - Ki67
KW - PIP3
KW - Polyethylene glycol (PEG)-fibrinogen hydrogels
UR - http://www.scopus.com/inward/record.url?scp=84887995600&partnerID=8YFLogxK
U2 - 10.1007/s10815-013-0052-8
DO - 10.1007/s10815-013-0052-8
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AN - SCOPUS:84887995600
SN - 1058-0468
VL - 30
SP - 1279
EP - 1288
JO - Journal of Assisted Reproduction and Genetics
JF - Journal of Assisted Reproduction and Genetics
IS - 10
ER -