@article{1f547f8cca5944cf9d41d4188d210bfc,
title = "Assay of (2′-5′)-Oligo(A) Synthesized in Vitro and the Analysis of Naturally Occurring (2′-5′)-Oligo(A) from Intact Cells",
abstract = "This chapter presents methods used for radioactive ATP incorporation and biological assays. The wide distribution of the 2-5A synthetase in a variety of cells and tissues and its variation with growth and hormone status have emphasized the wider possible significance of the 2-5A system and the desirability of assay methods for the routine estimation of naturally occurring preformed 2-5A. Accordingly, the methods that have been used successfully in the initial experiments on the recovery of 2-5A from intact cells are also presented. The 2-5A is effective at nanomolar concentrations in cell-free systems. The detection of 2-5A at concentrations lower than this has been complicated by the nonspecific inhibitory effects of the crude extracts in the biological assay employed, the sensitivity of the assays, and the amounts of starting material readily available. However, radioimmune and radiobinding assays are greatly facilitating analyses of this type. Naturally occurring 2-5A can be detected in crude trichloroacetic acid-soluble extracts, which are simple to prepare from intact cells.",
author = "Williams, {B. R.G.} and Brown, {R. E.} and Gilbert, {C. S.} and Golgher, {R. R.} and Wreschner, {D. H.} and Roberts, {W. K.} and Silverman, {R. H.} and Kerr, {Ian M.}",
note = "Funding Information: R. R. G. was the recipient of a Fellowship from the Conselho National de Desenvol-vimento Cientifico e Tecnologico, Brazil; and W. K. R. of a grant from the American Cancer Society.",
year = "1981",
month = jan,
day = "1",
doi = "10.1016/S0076-6879(81)79030-X",
language = "אנגלית",
volume = "79",
pages = "199--208",
journal = "Methods in Enzymology",
issn = "0076-6879",
publisher = "Academic Press Inc.",
number = "C",
}