Aromatic aldehyde and hydrazine activated peptide coated quantum dots for easy bioconjugation and live cell imaging

Gopal Iyer, Fabien Pinaud, Jianmin Xu, Yuval Ebenstein, Jack Li, Jessica Chang, Maxime Dahan, Shimon Weiss

Research output: Contribution to journalArticlepeer-review

Abstract

We present a robust scheme for preparation of semiconductor quantum dots (QDs) and cognate partners in a conjugation ready format. Our approach is based on bis-aryl hydrazone bond formation mediated by aromatic aldehyde and hydrazinonicotinate acetone hydrazone (HyNic) activated peptide coated quantum dots. We demonstrate controlled preparation of antibody-QD bioconjugates for specific targeting of endogenous epidermal growth factor receptors in breast cancer cells and for single QD tracking of transmembrane proteins via an extracellular epitope. The same approach was also used for optical mapping of RNA polymerases bound to combed genomic DNA in vitro.

Original languageEnglish
Pages (from-to)1006-1011
Number of pages6
JournalBioconjugate Chemistry
Volume22
Issue number6
DOIs
StatePublished - 15 Jun 2011
Externally publishedYes

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