TY - JOUR
T1 - Arachidonic acid metabolism in human granulosa cells
T2 - Evidence for cyclooxygenase and lipoxygenase activity in vitro
AU - Feldman, E.
AU - Haberman, S.
AU - Abisogun, A. O.
AU - Reich, R.
AU - Levran, D.
AU - Maschiach, S.
AU - Zuckermann, H.
AU - Rudak, E.
AU - Dor, J.
AU - Tsafriri, A.
N1 - Funding Information:
We thank Mrs R.SIager for her skillful technical assistance, Mrs M.Kopelowitz for her able secretarial help, Dr Y.Rokach of the Merck Frosst Canada, Inc., Dorval, Quebec, for the HETEs and leukotriene standards, Dr S.Hammarstrom, Karolinska Institute, Stockholm, Sweden, for 5,8,11-ETYA and Dr M.Nishikawa, of Takeda Chemical Industries, Ltd., Osaka, Japan for AA861. This investigation was supported by the Rockefeller Foundation, New York.
PY - 1986/9
Y1 - 1986/9
N2 - In view of the studies demonstrating the involvement of eicosanoids (prostaglandins and hydroxyperoxides, including leukotrienes) in ovulation in several mammalian species, we have examined the activity of the two enzyme systems, lipoxygenase and cyclooxygenase in human granulosa cells obtained from women undergoing in-vitro fertilization-embryo transfer. The activity of cyclooxygenase was assessed by radio-immunoassay of prostaglandin E and of 6-keto-prostaglan-din F1α, the conversion product of prostacyclin, accumulated in the culture medium of granulosa cells. Lipoxygenase activity was detected by the conversion of [14C]arachidonic acid into its products (hydroxyperoxides and leukotrienes) separated by reversed phase high-performance liquid chromatography. The results confirmed the activity of cyclooxygenase in human granulosa cells, production in vitro of prostaglandin E and prostacyclin and demonstrated the presence of active lipoxygenase enzymes. These results support the possible involvement of eicosanoids in ovulation of the human.
AB - In view of the studies demonstrating the involvement of eicosanoids (prostaglandins and hydroxyperoxides, including leukotrienes) in ovulation in several mammalian species, we have examined the activity of the two enzyme systems, lipoxygenase and cyclooxygenase in human granulosa cells obtained from women undergoing in-vitro fertilization-embryo transfer. The activity of cyclooxygenase was assessed by radio-immunoassay of prostaglandin E and of 6-keto-prostaglan-din F1α, the conversion product of prostacyclin, accumulated in the culture medium of granulosa cells. Lipoxygenase activity was detected by the conversion of [14C]arachidonic acid into its products (hydroxyperoxides and leukotrienes) separated by reversed phase high-performance liquid chromatography. The results confirmed the activity of cyclooxygenase in human granulosa cells, production in vitro of prostaglandin E and prostacyclin and demonstrated the presence of active lipoxygenase enzymes. These results support the possible involvement of eicosanoids in ovulation of the human.
UR - http://www.scopus.com/inward/record.url?scp=0023032366&partnerID=8YFLogxK
U2 - 10.1093/oxfordjournals.humrep.a136422
DO - 10.1093/oxfordjournals.humrep.a136422
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AN - SCOPUS:0023032366
VL - 1
SP - 353
EP - 356
JO - Human Reproduction
JF - Human Reproduction
SN - 0268-1161
IS - 6
ER -