TY - JOUR
T1 - Arabidopsis eIF3e interacts with subunits of the ribosome, Cop9 signalosome and proteasome
AU - Paz-Aviram, Tal
AU - Yahalom, Avital
AU - Chamovitz, Daniel A.
N1 - Funding Information:
the interaction of eIF3e with degradation of specific cell cynot the proteasome arises from eIF3e being cle regulators, it is also conceivable, that a proteasome substrate.27 Acknowledgements This researchD waos supported by Research Grant Award No. IS-3901-06c from BARD, The United States-Israel Binational Agricultural Research and Development Fund.
PY - 2008/6
Y1 - 2008/6
N2 - The roles of individual Eukaryotic translation Initiation Factor 3 (eIF3) subunits are largely unclear. Though some are essential, while others are thought to have regulatory roles. The "e" subunit, also known as Int-6, is a candidate for a regulatory subunit as it is not essential for translation initiation in yeasts. To further elucidate the roles of eIF3e, we have employed an interaction-trap screen using the yeast two-hybrid system. eIF3e interacts in yeast with subunits of the ribosome, COP9 signalosome and 26S proteasome. These interactions mesh well with our recent results which showed that eIF3e is degraded in a CSN-dependent, proteasome-dependent fashion, and inhibits translation when present in excess.
AB - The roles of individual Eukaryotic translation Initiation Factor 3 (eIF3) subunits are largely unclear. Though some are essential, while others are thought to have regulatory roles. The "e" subunit, also known as Int-6, is a candidate for a regulatory subunit as it is not essential for translation initiation in yeasts. To further elucidate the roles of eIF3e, we have employed an interaction-trap screen using the yeast two-hybrid system. eIF3e interacts in yeast with subunits of the ribosome, COP9 signalosome and 26S proteasome. These interactions mesh well with our recent results which showed that eIF3e is degraded in a CSN-dependent, proteasome-dependent fashion, and inhibits translation when present in excess.
KW - COP9 signalosome
KW - Int6
KW - Proteasome
KW - eIF3
KW - eIF3e
UR - http://www.scopus.com/inward/record.url?scp=44649197065&partnerID=8YFLogxK
U2 - 10.4161/psb.3.6.5434
DO - 10.4161/psb.3.6.5434
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AN - SCOPUS:44649197065
SN - 1559-2316
VL - 3
SP - 409
EP - 411
JO - Plant Signaling and Behavior
JF - Plant Signaling and Behavior
IS - 6
ER -