Apoptosis as an indicator for steroid sensitivity of lymphocytes in B-chronic lymphocytic leukemia

V. Vishlitzky, R. Ness-Abramof, R. Zemer, M. Ellis, M. Lishner, Y. Manor, M. S. Yuklea, A. Klein*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Apoptosis, programmed cell death, occurs in a variety of cellular systems and in response to many different stimuli. One group of apoptosis inducers are glucocorticosteroids which are also found in the battery of cytotoxic drugs used to treat CLL. In the present study we have examined the potency of the glucocorticosteroid-dexamethasone to induce apoptosis in lymphocytes of patients with B-CLL. Lymphocytes of 15 nontreated patients and 5 controls were isolated and incubated for 24 h in the presence or absence of dexamethasone (2 μM). Following incubation the cells were harvested and their DNA extracted. The extracted DNA samples were analysed for internucleosomal DNA cleavage by UV illumination after electrophoresis on agarose slab gel containing ethidium bromide. Five patients showed neither spontaneous nor dexamethasone induced apoptosis. Whereas, 10 patients, showed a dexamethasone-non-dependent spontaneous apoptosis which appeared 24 h after the start of incubation. The cells of these patients were the only ones to respond to dexamethasone showing an enhanced apoptosis effect. This study shows that apoptosis monitoring in CLL may provide important information regarding susceptibility of the cells to steroid induced apoptosis.

Original languageEnglish
Pages (from-to)461-463
Number of pages3
JournalOncology Reports
Volume2
Issue number3
DOIs
StatePublished - 1995
Externally publishedYes

Keywords

  • Apoptosis
  • Lymphocytic leukemia
  • Steroid sesnsitivity

Fingerprint

Dive into the research topics of 'Apoptosis as an indicator for steroid sensitivity of lymphocytes in B-chronic lymphocytic leukemia'. Together they form a unique fingerprint.

Cite this