Antimicrobial resistance of Clostridium difficile isolates in a tertiary medical center, Israel

Jihad Bishara*, Yoram Bloch, Moshe Garty, Jaqueline Behor, Zmira Samra

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The antimicrobial susceptibilities of 49 Clostridium difficile isolates obtained from patients with C. difficile-associated diarrhea to metronidazole, vancomycin, rifampicin, fusidic acid, doxycycline, and linezolid were determined by the disc diffusion and Etest (Biodisk, Solna, Sweden). Random amplification of polymorphic DNA-PCR amplification assay was performed for studying clonality of isolates. Resistance to metronidazole was found in 2% (1/49 isolates; MIC ≥ 256 μg/mL) of isolates and resistance to linezolid in 2% (1/49 isolates; MIC = 24 μg/mL). One isolate showed combined resistance to fusidic acid (by disc diffusion test) and rifampicin (MIC ≥ 32 μg/mL). All isolates were sensitive to doxycycline and vancomycin. Molecular typing revealed an absence of clonality among the resistant isolates, whereas the sensitive isolates were monoclonal. Resistance of C. difficile to metronidazole and other antimicrobials including linezolid exists in our institution. This finding should promote exploration of this problem in Israel and clarify the impact of resistance on outcome.

Original languageEnglish
Pages (from-to)141-144
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume54
Issue number2
DOIs
StatePublished - Feb 2006

Keywords

  • Clostridium difficile
  • Israel
  • Linezolid
  • Metronidazole
  • Resistance

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