Antifungal susceptibility profiles of rare ascomycetous yeasts

Antonio Pérez-Hansen; Cornelia Lass-Flörl; Michaela Lackner; M. Aigner; A. Alastruey-Izquierdo; S. Arikan-Akdagli; O. Bader; K. Becker; T. Boekhout; W. Buzina; O. A. Cornely; P. Hamal; S. E. Kidd; O. Kurzai; K. Lagrou; A. Lopes Colombo; M. Mares; H. Masoud; J. F. Meis; S. Oliveri; A. C. Rodloff; D. Orth-Höller; I. Guerrero-Lozano; M. Sanguinetti; E. Segal; S. J. Taj-Aldeen; A. M. Tortorano; L. Trovato; G. Walther; B. Willinger

doi:10.1093/jac/dkz231

Antifungal susceptibility profiles of rare ascomycetous yeasts

Antonio Pérez-Hansen, Cornelia Lass-Flörl, Michaela Lackner^*, M. Aigner, A. Alastruey-Izquierdo, S. Arikan-Akdagli, O. Bader, K. Becker, T. Boekhout, W. Buzina, O. A. Cornely, P. Hamal, S. E. Kidd, O. Kurzai, K. Lagrou, A. Lopes Colombo, M. Mares, H. Masoud, J. F. Meis, S. OliveriA. C. Rodloff, D. Orth-Höller, I. Guerrero-Lozano, M. Sanguinetti, E. Segal, S. J. Taj-Aldeen, A. M. Tortorano, L. Trovato, G. Walther, B. Willinger

^*Corresponding author for this work

Clinical Microbiology and Immunology

Innsbruck Medical University

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Objectives: To generate antifungal susceptibility patterns for Trichomonascus ciferrii (Candida ciferrii), Candida inconspicua (Torulopsis inconspicua) and Diutina rugosa species complex (Candida rugosa species complex), and to provide key parameters such as MIC₅₀, MIC₉₀ and tentative epidemiological cut-off values (TECOFFs). Methods: Our strain set included isolates of clinical origin: C. inconspicua (n=168), D. rugosa species complex (n=90) [Candida pararugosa (n=60), D. rugosa (n=26) and Candida mesorugosa (n=4)], Pichia norvegensis (Candida norvegensis) (n=15) and T. ciferrii (n=8). Identification was performed by MALDI-TOF MS or internal transcribed spacer sequencing. Antifungal susceptibility patterns were generated for azoles, echinocandins and amphotericin B using commercial Etest and the EUCAST broth microdilution method v7.3.1. Essential agreement (EA) was calculated for Etest and EUCAST. Results: C. inconspicua, C. pararugosa and P. norvegensis showed elevated azole MICs (MIC₅₀ ≥0.06 mg/L), and D. rugosa and C. pararugosa elevated echinocandin MICs (MIC₅₀ ≥0.06 mg/L). EA between methods was generally low (<90%); EA averaged 77.45%. TECOFFs were suggested for C. inconspicua and D. rugosa species complex. Conclusions: Rare yeast species tested shared high fluconazole MICs. D. rugosa species complex displayed high echinocandin MICs, while C. inconspicua and P. norvegensis were found to have high azole MICs. Overall, the agreement between EUCAST and Etest was poor and therefore MIC values generated with Etest cannot be directly compared with EUCAST results.

Original language	English
Pages (from-to)	2649-2656
Number of pages	8
Journal	Journal of Antimicrobial Chemotherapy
Volume	74
Issue number	9
DOIs	https://doi.org/10.1093/jac/dkz231
State	Published - 1 Sep 2019

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Pérez-Hansen, A., Lass-Flörl, C., Lackner, M., Aigner, M., Alastruey-Izquierdo, A., Arikan-Akdagli, S., Bader, O., Becker, K., Boekhout, T., Buzina, W., Cornely, O. A., Hamal, P., Kidd, S. E., Kurzai, O., Lagrou, K., Lopes Colombo, A., Mares, M., Masoud, H., Meis, J. F., ... Willinger, B. (2019). Antifungal susceptibility profiles of rare ascomycetous yeasts. Journal of Antimicrobial Chemotherapy, 74(9), 2649-2656. https://doi.org/10.1093/jac/dkz231

@article{dc7052ea91db4129a2b9c1534f3829e4,

title = "Antifungal susceptibility profiles of rare ascomycetous yeasts",

abstract = "Objectives: To generate antifungal susceptibility patterns for Trichomonascus ciferrii (Candida ciferrii), Candida inconspicua (Torulopsis inconspicua) and Diutina rugosa species complex (Candida rugosa species complex), and to provide key parameters such as MIC50, MIC90 and tentative epidemiological cut-off values (TECOFFs). Methods: Our strain set included isolates of clinical origin: C. inconspicua (n=168), D. rugosa species complex (n=90) [Candida pararugosa (n=60), D. rugosa (n=26) and Candida mesorugosa (n=4)], Pichia norvegensis (Candida norvegensis) (n=15) and T. ciferrii (n=8). Identification was performed by MALDI-TOF MS or internal transcribed spacer sequencing. Antifungal susceptibility patterns were generated for azoles, echinocandins and amphotericin B using commercial Etest and the EUCAST broth microdilution method v7.3.1. Essential agreement (EA) was calculated for Etest and EUCAST. Results: C. inconspicua, C. pararugosa and P. norvegensis showed elevated azole MICs (MIC50 ≥0.06 mg/L), and D. rugosa and C. pararugosa elevated echinocandin MICs (MIC50 ≥0.06 mg/L). EA between methods was generally low (<90%); EA averaged 77.45%. TECOFFs were suggested for C. inconspicua and D. rugosa species complex. Conclusions: Rare yeast species tested shared high fluconazole MICs. D. rugosa species complex displayed high echinocandin MICs, while C. inconspicua and P. norvegensis were found to have high azole MICs. Overall, the agreement between EUCAST and Etest was poor and therefore MIC values generated with Etest cannot be directly compared with EUCAST results.",

author = "Antonio P{\'e}rez-Hansen and Cornelia Lass-Fl{\"o}rl and Michaela Lackner and M. Aigner and A. Alastruey-Izquierdo and S. Arikan-Akdagli and O. Bader and K. Becker and T. Boekhout and W. Buzina and Cornely, {O. A.} and P. Hamal and Kidd, {S. E.} and O. Kurzai and K. Lagrou and {Lopes Colombo}, A. and M. Mares and H. Masoud and Meis, {J. F.} and S. Oliveri and Rodloff, {A. C.} and D. Orth-H{\"o}ller and I. Guerrero-Lozano and M. Sanguinetti and E. Segal and Taj-Aldeen, {S. J.} and Tortorano, {A. M.} and L. Trovato and G. Walther and B. Willinger",

year = "2019",

month = sep,

day = "1",

doi = "10.1093/jac/dkz231",

language = "אנגלית",

volume = "74",

pages = "2649--2656",

journal = "Journal of Antimicrobial Chemotherapy",

issn = "0305-7453",

publisher = "Oxford University Press",

number = "9",

}

Pérez-Hansen, A, Lass-Flörl, C, Lackner, M, Aigner, M, Alastruey-Izquierdo, A, Arikan-Akdagli, S, Bader, O, Becker, K, Boekhout, T, Buzina, W, Cornely, OA, Hamal, P, Kidd, SE, Kurzai, O, Lagrou, K, Lopes Colombo, A, Mares, M, Masoud, H, Meis, JF, Oliveri, S, Rodloff, AC, Orth-Höller, D, Guerrero-Lozano, I, Sanguinetti, M, Segal, E, Taj-Aldeen, SJ, Tortorano, AM, Trovato, L, Walther, G & Willinger, B 2019, 'Antifungal susceptibility profiles of rare ascomycetous yeasts', Journal of Antimicrobial Chemotherapy, vol. 74, no. 9, pp. 2649-2656. https://doi.org/10.1093/jac/dkz231

TY - JOUR

T1 - Antifungal susceptibility profiles of rare ascomycetous yeasts

AU - Pérez-Hansen, Antonio

AU - Lass-Flörl, Cornelia

AU - Lackner, Michaela

AU - Aigner, M.

AU - Alastruey-Izquierdo, A.

AU - Arikan-Akdagli, S.

AU - Bader, O.

AU - Becker, K.

AU - Boekhout, T.

AU - Buzina, W.

AU - Cornely, O. A.

AU - Hamal, P.

AU - Kidd, S. E.

AU - Kurzai, O.

AU - Lagrou, K.

AU - Lopes Colombo, A.

AU - Mares, M.

AU - Masoud, H.

AU - Meis, J. F.

AU - Oliveri, S.

AU - Rodloff, A. C.

AU - Orth-Höller, D.

AU - Guerrero-Lozano, I.

AU - Sanguinetti, M.

AU - Segal, E.

AU - Taj-Aldeen, S. J.

AU - Tortorano, A. M.

AU - Trovato, L.

AU - Walther, G.

AU - Willinger, B.

PY - 2019/9/1

Y1 - 2019/9/1

N2 - Objectives: To generate antifungal susceptibility patterns for Trichomonascus ciferrii (Candida ciferrii), Candida inconspicua (Torulopsis inconspicua) and Diutina rugosa species complex (Candida rugosa species complex), and to provide key parameters such as MIC50, MIC90 and tentative epidemiological cut-off values (TECOFFs). Methods: Our strain set included isolates of clinical origin: C. inconspicua (n=168), D. rugosa species complex (n=90) [Candida pararugosa (n=60), D. rugosa (n=26) and Candida mesorugosa (n=4)], Pichia norvegensis (Candida norvegensis) (n=15) and T. ciferrii (n=8). Identification was performed by MALDI-TOF MS or internal transcribed spacer sequencing. Antifungal susceptibility patterns were generated for azoles, echinocandins and amphotericin B using commercial Etest and the EUCAST broth microdilution method v7.3.1. Essential agreement (EA) was calculated for Etest and EUCAST. Results: C. inconspicua, C. pararugosa and P. norvegensis showed elevated azole MICs (MIC50 ≥0.06 mg/L), and D. rugosa and C. pararugosa elevated echinocandin MICs (MIC50 ≥0.06 mg/L). EA between methods was generally low (<90%); EA averaged 77.45%. TECOFFs were suggested for C. inconspicua and D. rugosa species complex. Conclusions: Rare yeast species tested shared high fluconazole MICs. D. rugosa species complex displayed high echinocandin MICs, while C. inconspicua and P. norvegensis were found to have high azole MICs. Overall, the agreement between EUCAST and Etest was poor and therefore MIC values generated with Etest cannot be directly compared with EUCAST results.

AB - Objectives: To generate antifungal susceptibility patterns for Trichomonascus ciferrii (Candida ciferrii), Candida inconspicua (Torulopsis inconspicua) and Diutina rugosa species complex (Candida rugosa species complex), and to provide key parameters such as MIC50, MIC90 and tentative epidemiological cut-off values (TECOFFs). Methods: Our strain set included isolates of clinical origin: C. inconspicua (n=168), D. rugosa species complex (n=90) [Candida pararugosa (n=60), D. rugosa (n=26) and Candida mesorugosa (n=4)], Pichia norvegensis (Candida norvegensis) (n=15) and T. ciferrii (n=8). Identification was performed by MALDI-TOF MS or internal transcribed spacer sequencing. Antifungal susceptibility patterns were generated for azoles, echinocandins and amphotericin B using commercial Etest and the EUCAST broth microdilution method v7.3.1. Essential agreement (EA) was calculated for Etest and EUCAST. Results: C. inconspicua, C. pararugosa and P. norvegensis showed elevated azole MICs (MIC50 ≥0.06 mg/L), and D. rugosa and C. pararugosa elevated echinocandin MICs (MIC50 ≥0.06 mg/L). EA between methods was generally low (<90%); EA averaged 77.45%. TECOFFs were suggested for C. inconspicua and D. rugosa species complex. Conclusions: Rare yeast species tested shared high fluconazole MICs. D. rugosa species complex displayed high echinocandin MICs, while C. inconspicua and P. norvegensis were found to have high azole MICs. Overall, the agreement between EUCAST and Etest was poor and therefore MIC values generated with Etest cannot be directly compared with EUCAST results.

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U2 - 10.1093/jac/dkz231

DO - 10.1093/jac/dkz231

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SN - 0305-7453

VL - 74

SP - 2649

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JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

IS - 9

ER -

Antifungal susceptibility profiles of rare ascomycetous yeasts

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