Antifreeze protein suppresses spontaneous neural activity and protects neurons from hypothermia/re-warming injury

Liel Rubinsky; Nadav Raichman; Jacob Lavee; Hanan Frenk; Eshel Ben-Jacob; Philip E. Bickler

doi:10.1016/j.neures.2010.04.004

Antifreeze protein suppresses spontaneous neural activity and protects neurons from hypothermia/re-warming injury

Liel Rubinsky^*, Nadav Raichman, Jacob Lavee, Hanan Frenk, Eshel Ben-Jacob, Philip E. Bickler

^*Corresponding author for this work

School of Psychological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Antifreeze proteins (AFP) are associated with protection from freezing. We measured the effect of type I antifreeze protein on spontaneous bursting of mixed neuronal/glial cultures using a multi-electrode array culture system. Antifreeze protein (10. mg/ml) reversibly depressed bursting activity without inhibiting mitochondrial oxidative capacity. The effect of antifreeze protein on cold/re-warming injury was investigated in rat hippocampal slice cultures. Compared to bovine serum albumin at a similar concentration, antifreeze protein protected hippocampal neurons from 8. h of profound hypothermia at (4°C) followed by re-warming. The protection observed is believed to be associated with the inhibitory effect of antifreeze protein.

Original language	English
Pages (from-to)	256-259
Number of pages	4
Journal	Neuroscience Research
Volume	67
Issue number	3
DOIs	https://doi.org/10.1016/j.neures.2010.04.004
State	Published - Jul 2010

Keywords

Antifreeze proteins
Hypothermia
Ion channels
Neural network dynamics
Neurons
Neuroprotection

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10.1016/j.neures.2010.04.004

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abstract = "Antifreeze proteins (AFP) are associated with protection from freezing. We measured the effect of type I antifreeze protein on spontaneous bursting of mixed neuronal/glial cultures using a multi-electrode array culture system. Antifreeze protein (10. mg/ml) reversibly depressed bursting activity without inhibiting mitochondrial oxidative capacity. The effect of antifreeze protein on cold/re-warming injury was investigated in rat hippocampal slice cultures. Compared to bovine serum albumin at a similar concentration, antifreeze protein protected hippocampal neurons from 8. h of profound hypothermia at (4°C) followed by re-warming. The protection observed is believed to be associated with the inhibitory effect of antifreeze protein.",

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AU - Ben-Jacob, Eshel

AU - Bickler, Philip E.

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Antifreeze protein suppresses spontaneous neural activity and protects neurons from hypothermia/re-warming injury

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Keywords

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