Antifreeze protein suppresses spontaneous neural activity and protects neurons from hypothermia/re-warming injury

Liel Rubinsky*, Nadav Raichman, Jacob Lavee, Hanan Frenk, Eshel Ben-Jacob, Philip E. Bickler

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Antifreeze proteins (AFP) are associated with protection from freezing. We measured the effect of type I antifreeze protein on spontaneous bursting of mixed neuronal/glial cultures using a multi-electrode array culture system. Antifreeze protein (10. mg/ml) reversibly depressed bursting activity without inhibiting mitochondrial oxidative capacity. The effect of antifreeze protein on cold/re-warming injury was investigated in rat hippocampal slice cultures. Compared to bovine serum albumin at a similar concentration, antifreeze protein protected hippocampal neurons from 8. h of profound hypothermia at (4°C) followed by re-warming. The protection observed is believed to be associated with the inhibitory effect of antifreeze protein.

Original languageEnglish
Pages (from-to)256-259
Number of pages4
JournalNeuroscience Research
Volume67
Issue number3
DOIs
StatePublished - Jul 2010

Funding

FundersFunder number
National Institute of General Medical SciencesR01GM052212

    Keywords

    • Antifreeze proteins
    • Hypothermia
    • Ion channels
    • Neural network dynamics
    • Neurons
    • Neuroprotection

    Fingerprint

    Dive into the research topics of 'Antifreeze protein suppresses spontaneous neural activity and protects neurons from hypothermia/re-warming injury'. Together they form a unique fingerprint.

    Cite this