Antibody‐dependent Cell‐mediated Cytotoxicity (ADCC) of Penicillin‐treated Human Red Blood Cells

Israel Yust*, B. Frisch, Naomi Goldsher

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Summary. Penicillin‐treated human red blood cells (RBC) were lysed by the cooperation of autologous nonsensitized peripheral blood mononuclear cells and human anti‐penicillin serum. Using a rapid (3 h) assay of antibody‐dependent cell‐mediated cytotoxicity (ADGC), lysis was proportional to serum (anti‐penicillin antibody) concentration, to incubation time and to the concentration of attacking cells, which were obtained from normal human peripheral blood by Ficoll‐Hypaque separation. Incubation of these lymphoid effector cells on a nylon column prior to the tests depleted the number of phagocytic (latex positive) cells in the effluent; there was a concomitant drop in cytotoxic activity. Enrichment of mononuclear phagocytes in the attacking cell population by albumin gradient separation led to an increase in cytotoxicity. Granulocytes separated by Ficoll‐Hypaque were not active in this system. Using specific antisera the antibody was found to be of the IgG1 sub‐class. Anti‐penicillin antibody activated the complement system in vitro, but failed to induce lysis of penicillin‐treated RBC in the presence of complement without attacking cells. These results suggest that ADCC may participate in the destruction of RBC in penicillin‐induced haemolysis in vivo.

Original languageEnglish
Pages (from-to)443-452
Number of pages10
JournalBritish Journal of Haematology
Volume47
Issue number3
DOIs
StatePublished - Mar 1981
Externally publishedYes

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