@inbook{8a903c59c6db47939d6bc5c1ddd85ee2,
title = "Antibody-driven proximity labeling in fixed tissues",
abstract = "Protein function often depends on assemblies and interactions. These show complex spatial and temporal organization within the cell. Analysis of protein function can be greatly assisted by grouping proteins with their neighbors. Rather than relying on affinity, proximity labeling targets proteins proximal to the target of interest. We describe a protocol for antibody-guided deposition of tags in fixed and permeabilized cell lines and primary human tissue samples.",
keywords = "Antibody, Biotin, HRP, Mass-spectrometry, Protein-protein interactions, Proximity labeling",
author = "Bar, {Daniel Z.} and Collins, {Francis S.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-9537-0_6",
language = "אנגלית",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "73--81",
booktitle = "Methods in Molecular Biology",
}