TY - JOUR
T1 - Antibodies to muscarinic acetylcholine receptors in myasthenia gravis
AU - Michaelson, Daniel M.
AU - Korczyn, Amos D.
AU - Sokolovsky, Mordechai
N1 - Funding Information:
Acknowledgement This work was supported in part by a grant to D.M.M. from the Muscular Dystrophy Association and by the Recanati Foundation for Medical Research (Israel). We are grateful to Mrs. Ronit Galron for excellent technical assistance and to Dr. T. Treves for providing the sera.
PY - 1982/1/15
Y1 - 1982/1/15
N2 - IgG obtained from patients with myasthenia gravis block the specific binding of the muscarinic antagonists (3H)-N-methyl-4-piperidyl benzilate (4NMPB) and (3H)-Quinuclidinyl benzilate to rat brain muscarinic acetylcholine receptors. IgG obtained from healthy controls have a much smaller effect. The inhibitory effect of the myasthenic IgG increases linearly with immunoglobulin concentration and has no effect on the affinity of the muscarinic receptors towards (3H)-4NMPB (KD = 0.7 ± 0.1 nM). This implies that the inhibition is probably due to the blocking of some of the muscarinic receptors by the myasthenic IgG, and not due to alteration in affinity of all the receptors. it remains to be established whether the presence of antimuscarinic receptor activity in the serum of myasthenic patients is of importance in the pathophysiology and diagnosis of myasthenia gravis.
AB - IgG obtained from patients with myasthenia gravis block the specific binding of the muscarinic antagonists (3H)-N-methyl-4-piperidyl benzilate (4NMPB) and (3H)-Quinuclidinyl benzilate to rat brain muscarinic acetylcholine receptors. IgG obtained from healthy controls have a much smaller effect. The inhibitory effect of the myasthenic IgG increases linearly with immunoglobulin concentration and has no effect on the affinity of the muscarinic receptors towards (3H)-4NMPB (KD = 0.7 ± 0.1 nM). This implies that the inhibition is probably due to the blocking of some of the muscarinic receptors by the myasthenic IgG, and not due to alteration in affinity of all the receptors. it remains to be established whether the presence of antimuscarinic receptor activity in the serum of myasthenic patients is of importance in the pathophysiology and diagnosis of myasthenia gravis.
UR - http://www.scopus.com/inward/record.url?scp=0020056658&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(82)91939-8
DO - 10.1016/0006-291X(82)91939-8
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AN - SCOPUS:0020056658
SN - 0006-291X
VL - 104
SP - 52
EP - 57
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -