Antibodies Against Glycoprotein 2 Are Specific Biomarkers for Pediatric Crohn’s Disease

Michael Shpoliansky, Dirk Roggenbuck, Marina Pinsker, Naomi Salamon, Batia Weiss, Dror S. Shouval, Lael Werner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Background: Serological markers can assist in accurate differentiation between Crohn’s disease (CD) and ulcerative colitis (UC). One such marker is anti-glycoprotein 2 (anti-GP2) which was shown to be a specific marker for CD in adult patients. The aim of our study was to assess the utility of anti-GP2 and GP2 as biomarkers for pediatric CD, and determine whether they correlate with disease activity. Methods: Serum samples were tested by ELISA for anti-GP2 isoform 4 IgG and IgA, and also for GP2. Results were correlated with demographic and clinical data. Results: The cohort consisted of 53 pediatric patients with CD, 42 with UC, and 53 controls. Levels of anti-GP2 were significantly increased in pediatric patients with CD in comparison with patients with UC, and control subjects, with high positive predictive value for both IgG and IgA (97.9% and 82.6%, respectively). While specificity of anti-GP2 IgG and IgA was very high (98.7% and 90.0%, respectively), sensitivity was low (42.0% and 35.5% for IgG and IgA, respectively). In CD, anti-GP2 correlated with disease activity, and decreased in treatment-naïve patients following successful induction therapy. A higher IgA anti-GP2 was also demonstrated in patients with ileo-colonic involvement, and was associated with a younger age. Finally, positive GP2 level was identified in only 1/211 serum samples. Conclusions: A positive anti-GP2 level is highly associated with CD, while a negative result does not exclude CD. Additional studies are required to determine whether these markers can be used in pediatric patients with CD for risk stratification.

Original languageEnglish
Pages (from-to)2619-2626
Number of pages8
JournalDigestive Diseases and Sciences
Issue number8
StatePublished - Aug 2021


  • Anti-GP2
  • Biomarkers
  • Inflammatory bowel disease
  • PAB
  • Serology


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