Antibodies against glycoprotein 2 are novel markers of intestinal inflammation in patients with an ileal pouch

Lael Werner, Andreas Sturm, Dirk Roggenbuck, Lior Yahav, Tanir Zion, Elhanan Meirowithz, Amos Ofer, Hanan Guzner-Gur, Hagit Tulchinsky, Iris Dotan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background and aims: The Crohn's disease (CD)-specific pancreatic auto-antibodies (PAB), have been recently identified to target glycoprotein 2 (GP2). Pouchitis is an inflammation of the small bowel developing in up to 60% of ulcerative colitis patients undergoing proctocolectomy and ileal pouch anal anastomosis. Occurrence of CD-specific antibodies was reported to be a predictor of pouchitis. We aimed to assess the prevalence of anti-GP2 antibodies (anti-GP2) in the serum and feces of pouch patients and to correlate them with clinical parameters. Furthermore, we examined mucosal expression of the GP2 protein in the pouch. Methods: Pouch patients were prospectively recruited and checked for clinical, endoscopic, and laboratory markers of inflammation. IgG and IgA anti-GP2 levels in serum and fecal samples were determined using ELISA. GP2 protein was assessed by immunohistochemistry. Results: Anti-GP2 was elevated in both serum and fecal samples of patients with inflamed compared to those with non-inflamed pouches and patients with familial-adenomatous polyposis after surgery (p < 0.05, respectively). Moreover, patients with CD-like complications exhibited significantly higher anti-GP2 titers than those without CD-like complications (p ≤ 0.01). High levels of anti-GP2 correlated with more frequent bowel movements per day and with the presence of at least one anti-glycan antibody (p ≤ 0.05). GP2 itself was more abundant in the mucosa of patients with chronic pouchitis. Conclusions: Anti-GP2 exists in the serum and feces of pouch patients and correlates with pouch inflammation, and presence of other serological markers. Thus, anti-GP2 may contribute to better stratification of pouchitis, more-so when the inflammation exhibits CD-like complications.

Original languageEnglish
Pages (from-to)e522-e532
JournalJournal of Crohn's and Colitis
Volume7
Issue number11
DOIs
StatePublished - 2013

Keywords

  • Anti-GP2
  • Glycoprotein 2
  • IPAA
  • Pouchitis
  • Serological markers

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