Anti-TPO-mediated specific features of the placenta immunohistochemical profile and possible mechanisms for fetal loss

Elena Borodina*, Alexander M. Gzgzyan, Liailia Kh Dzhemlikhanova, Dariko A. Niauri, Gulrukhsor Kh Tolibova, Tatiana G. Tral, Igor Y. Kogan, Galina Kh Safarian, Yuri Ostrinski, Leonid P. Churilov, Howard Amital, Miri Blank, Yehuda Shoenfeld

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Passive transfer of antithyroid antibodies in mice leads to reproductive disorders. The purpose was to assess the placental tissue of experimental animals under the influence of the circulating thyroperoxidase antibodies. We performed an immunohistochemical examination of murine placentae after a passive transfer of thyroperoxidase antibodies. Placentae of mice that passively transferred IgG from healthy donors were used as control samples. For histological examination, 30 placental samples were selected from mice from the anti-TPO group and 40 placental samples were taken from mice from the IgG group. Immunostaining for VEGFR1, THBS 1, Laminin, CD31, CD34, FGF-β, CD56, CD14, TNF-α, kisspeptin, MCL 1, and Annexin V was performed. There is a significant decrease in the relative area of the expression of VEGFR1 (23.42 ± 0.85 vs. 33.44 ± 0.35, P < 0.01), thrombospondin 1 (31.29 ± 0.83 vs. 34.51 ± 0.75, P < 0.01), CD14 (25.80 ± 0.57 vs. 32.07 ± 0.36, P <. 01), CD56 (30.08 ± 0.90 vs. 34.92 ± 0.15, P < 0.01), kisspeptin (25.94 ± 0.47 vs. 31.27 ± 0.57, P < 0.01), MCL 1 (29.24 ± 1.06 vs. 38.57 ± 0.79, P < 0.01) in the labyrinth zone of the placentae of mice from the anti-TPO group compared with control group. A significant increase in the relative expression of laminin and FGF-β was noted in the group of mice to which antibodies to thyroperoxidase were transferred, compared with the control group (36.73 ± 1.38 vs. 29.83 ± 0.94, P < 0.01 and 23.26 ± 0.61 vs. 16.38 ± 1.01, P < 0.01respectively). Our study exposed an imbalance of pro- and anti-angiogenic factors, decreased representation of placental macrophages and NK cells, abnormal trophoblast invasion processes, and insufficient expression of antiapoptotic factors in the placentae of mice in which anti-TPO antibodies were passively transferred.

Original languageEnglish
Pages (from-to)235-242
Number of pages8
JournalClinical and Experimental Immunology
Volume213
Issue number2
DOIs
StatePublished - Aug 2023

Funding

FundersFunder number
Government Council on Grants, Russian Federation

    Keywords

    • FGF-β
    • VEGFR1
    • antithyroid antibodies
    • fetal loss
    • placenta

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