Anti-T lymphocyte globulin (ATG) induces generation of regulatory T cells, at least part of them express activated CD44

Orly Shimony, Arnon Nagler, Yechiel N. Gellman, Efrat Refaeli, Nir Rosenblum, Lora Eshkar-Sebban, Ronit Yerushalmi, Avichai Shimoni, Simon D. Lytton, Anfisa Stanevsky, Reuven Or, David Naor

Research output: Contribution to journalArticlepeer-review

Abstract

We show here that the anti-T lymphocyte immunoglobulin (ATG) can induce Treg cells following 24-h incubation in human peripheral blood mononuclear cells (PBMCs). The ATG-induced Treg cells express known cell surface markers (e.g., CD25, FoxP3) and suppress the proliferation of autologous responder PBMCs, stimulated with allogeneic PBMCs, when added into the mixed lymphocyte culture (MLC) at zero time point or 48 h later. We expanded the characteristics of the ATG-induced human Treg cells by showing that they express a novel biomarker designated "activated CD44". ATG-induced Treg cells retain their suppressor function after freezing and thawing or irradiation. Suppression of MLC by ATG-induced Treg cells is consistently seen when the Treg cells and the responder cells were derived from the same donor, but not when they derived from different donors. Finally, patients undergoing stem cell transplantation and conditioned with ATG generate in vivo Treg cells that suppress MLC.

Original languageEnglish
Pages (from-to)173-188
Number of pages16
JournalJournal of Clinical Immunology
Volume32
Issue number1
DOIs
StatePublished - Feb 2012
Externally publishedYes

Keywords

  • Activated CD44
  • FoxP3
  • Hematological diseases
  • Regulatory T cells

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