Anti-malarial drug targets: Screening for inhibitors of 2C-methyl-d-erythritol 4-phosphate synthase (IspC protein) in Mediterranean plants

J. Kaiser; M. Yassin; S. Prakash; N. Safi; M. Agami; S. Lauw; E. Ostrozhenkova; A. Bacher; F. Rohdich; W. Eisenreich; J. Safi; A. Golan-Goldhirsh

doi:10.1016/j.phymed.2006.12.018

Anti-malarial drug targets: Screening for inhibitors of 2C-methyl-d-erythritol 4-phosphate synthase (IspC protein) in Mediterranean plants

J. Kaiser, M. Yassin, S. Prakash, N. Safi, M. Agami, S. Lauw, E. Ostrozhenkova, A. Bacher, F. Rohdich, W. Eisenreich^*, J. Safi, A. Golan-Goldhirsh

^*Corresponding author for this work

School of Plant Sciences and Food Security

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

The recently discovered non-mevalonate pathway of isoprenoid biosynthesis serves as the unique source of terpenoids in numerous pathogenic eubacteria and in apicoplast-type protozoa, most notably Plasmodium, but is absent in mammalian cells. It is therefore an attractive target for anti-infective chemotherapy. The first committed step of the non-mevalonate pathway is catalyzed by 2C-methyl-d-erythritol 4-phosphate synthase (IspC). Using photometric and NMR spectroscopic assays, we screened extracts of Mediterranean plants for inhibitors of the enzyme. Strongest inhibitory activity was found in leaf extracts of Cercis siliquastrum.

Original language	English
Pages (from-to)	242-249
Number of pages	8
Journal	Phytomedicine
Volume	14
Issue number	4
DOIs	https://doi.org/10.1016/j.phymed.2006.12.018
State	Published - 10 Apr 2007

Funding

Funders	Funder number
Deutsche Forschungsgemeinschaft

Keywords

Antibiotic
Cercis siliquastrum
Deoxyxylulose inhibitors of 2C-methyl-D-erythritol 4-phosphate sythase (IspCprotein)
Malaria
Terpene

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.phymed.2006.12.018

Cite this

Kaiser, J., Yassin, M., Prakash, S., Safi, N., Agami, M., Lauw, S., Ostrozhenkova, E., Bacher, A., Rohdich, F., Eisenreich, W., Safi, J., & Golan-Goldhirsh, A. (2007). Anti-malarial drug targets: Screening for inhibitors of 2C-methyl-d-erythritol 4-phosphate synthase (IspC protein) in Mediterranean plants. Phytomedicine, 14(4), 242-249. https://doi.org/10.1016/j.phymed.2006.12.018

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title = "Anti-malarial drug targets: Screening for inhibitors of 2C-methyl-d-erythritol 4-phosphate synthase (IspC protein) in Mediterranean plants",

abstract = "The recently discovered non-mevalonate pathway of isoprenoid biosynthesis serves as the unique source of terpenoids in numerous pathogenic eubacteria and in apicoplast-type protozoa, most notably Plasmodium, but is absent in mammalian cells. It is therefore an attractive target for anti-infective chemotherapy. The first committed step of the non-mevalonate pathway is catalyzed by 2C-methyl-d-erythritol 4-phosphate synthase (IspC). Using photometric and NMR spectroscopic assays, we screened extracts of Mediterranean plants for inhibitors of the enzyme. Strongest inhibitory activity was found in leaf extracts of Cercis siliquastrum.",

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author = "J. Kaiser and M. Yassin and S. Prakash and N. Safi and M. Agami and S. Lauw and E. Ostrozhenkova and A. Bacher and F. Rohdich and W. Eisenreich and J. Safi and A. Golan-Goldhirsh",

note = "Funding Information: This work was supported by the Deutsche Forschungsgemeinschaft. We also thank Mrs. Ulrike Stier (Bad Soden) and the Hans Fischer Gesellschaft e.V. for generous sponsoring of this research work. ",

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Kaiser, J, Yassin, M, Prakash, S, Safi, N, Agami, M, Lauw, S, Ostrozhenkova, E, Bacher, A, Rohdich, F, Eisenreich, W, Safi, J & Golan-Goldhirsh, A 2007, 'Anti-malarial drug targets: Screening for inhibitors of 2C-methyl-d-erythritol 4-phosphate synthase (IspC protein) in Mediterranean plants', Phytomedicine, vol. 14, no. 4, pp. 242-249. https://doi.org/10.1016/j.phymed.2006.12.018

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AU - Kaiser, J.

AU - Yassin, M.

AU - Prakash, S.

AU - Safi, N.

AU - Agami, M.

AU - Lauw, S.

AU - Ostrozhenkova, E.

AU - Bacher, A.

AU - Rohdich, F.

AU - Eisenreich, W.

AU - Safi, J.

AU - Golan-Goldhirsh, A.

N1 - Funding Information: This work was supported by the Deutsche Forschungsgemeinschaft. We also thank Mrs. Ulrike Stier (Bad Soden) and the Hans Fischer Gesellschaft e.V. for generous sponsoring of this research work.

PY - 2007/4/10

Y1 - 2007/4/10

N2 - The recently discovered non-mevalonate pathway of isoprenoid biosynthesis serves as the unique source of terpenoids in numerous pathogenic eubacteria and in apicoplast-type protozoa, most notably Plasmodium, but is absent in mammalian cells. It is therefore an attractive target for anti-infective chemotherapy. The first committed step of the non-mevalonate pathway is catalyzed by 2C-methyl-d-erythritol 4-phosphate synthase (IspC). Using photometric and NMR spectroscopic assays, we screened extracts of Mediterranean plants for inhibitors of the enzyme. Strongest inhibitory activity was found in leaf extracts of Cercis siliquastrum.

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KW - Antibiotic

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KW - Deoxyxylulose inhibitors of 2C-methyl-D-erythritol 4-phosphate sythase (IspCprotein)

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Anti-malarial drug targets: Screening for inhibitors of 2C-methyl-d-erythritol 4-phosphate synthase (IspC protein) in Mediterranean plants

Abstract

Funding

Keywords

UN SDGs

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