Anti-cancer binary system activated by bacteriophage HK022 integrase

Amer Elias, Natasha Gritsenko, Rena Gorovits, Itay Spector, Gali Prag, Ezra Yagil, Mikhail Kolot

Research output: Contribution to journalArticlepeer-review

Abstract

The binary system presented in this work is based on the bacteriophage HK022 integrase recombinase that activates the expression of a silenced Diphtheria toxin gene, both controlled by the cancer specific hTERT promoter. Using a lung cancer mice model, assays of different apoptotic and anti-apoptotic factors have demonstrated that the Integrase based binary system is highly specific towards cancer cells and more efficient compared to the conventional mono system whose toxin is directly expressed under hTERT. In a mice survival test, this binary system demonstrated longer persistence compared to the untreated and the mono treated ones. The reason underlying the advantage of this binary system over the mono system seems to be an overexpression of various hTERT suppressing factors induced by the mono system.

Original languageEnglish
Pages (from-to)27487-27501
Number of pages15
JournalOncotarget
Volume9
Issue number44
DOIs
StatePublished - 1 Jun 2018

Keywords

  • Cancer therapy
  • Coliphage HK022 integrase
  • DTA toxin
  • Lung cancer
  • Site-specific recombination

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